Project description:We infected cells expressing a FLAG-tagged EfAvs5 with phage T7, and immunoprecipitated the tagged EfAvs5 together with proteins bound to it. Mass spectrometry analysis showed 17 proteins of phage T7 were pulled down together with EfAvs5. These proteins are potential activators of EfAvs5.The control group is not infected by phage.
Project description:The global transcriptional profile of novel T7-like Pseudomonas aeruginosa phage LUZ100 was obtained using the long read RNA sequencing technique ONT-cappable-seq. Using this approach we obtained a comprehensive genome-wide map of viral transcription start sites, terminators and transcription units and gained new insights in the molecular mechanisms of transcriptional regulation of T7-like temperate phages.
