Project description:Microbes are an important trigger of chronic rhinosinusitis (CRS). Staphylococcus aureus is a CRS-related pathogen that disrupts the epithelial barrier and activates mucosal inflammation. Given the ineffectiveness of antimicrobial therapy in CRS, we explored a potential treatment based on the interaction of bacteria within the dysbiotic microenvironment of the nasal mucosa. Microbiome analyses of clinical isolates from CRS patients and healthy controls were employed to identify Corynebacterium striatum as a potential inhibitor of pathogenic S. aureus. A co-infection culture model using human nasal epithelial cells (hNECs) was constructed to investigate the inhibition of S. aureus-induced disruption of the host epithelium by C. striatum. Further analysis of the inhibition of S. aureus by C. striatum was carried out using a bacteria co-culture model.
Project description:Microbes are an important trigger of chronic rhinosinusitis (CRS). Staphylococcus aureus is a CRS-related pathogen that disrupts the epithelial barrier and activates mucosal inflammation. Given the ineffectiveness of antimicrobial therapy in CRS, we explored a potential treatment based on the interaction of bacteria within the dysbiotic microenvironment of the nasal mucosa. Microbiome analyses of clinical isolates from CRS patients and healthy controls were employed to identify Corynebacterium striatum as a potential inhibitor of pathogenic S. aureus. A co-infection culture model using human nasal epithelial cells (hNECs) was constructed to investigate the inhibition of S. aureus-induced disruption of the host epithelium by C. striatum. Further analysis of the inhibition of S. aureus by C. striatum was carried out using a bacteria co-culture model.
2026-03-01 | GSE284409 | GEO
Project description:Phylogenetic and phenotypic analysis of Corynebacterium jeikeium clinical isolates
Project description:Oligonucleotide DNA microarrays were used as a platform to compare C. jejuni isolates from feedlot cattle and human clinical cases from Alberta. Comparative genomic hybridization (CGH) analysis was performed on 87 isolates (46 bovine, 41 human) obtained within the same geographical regions and time frame. In addition, We also performed gene association analysis to determine if any genes may be differentially distributed between human and cattle sources or between clusters dominated by either human or cattle isolates (“human enriched” vs “cattle enriched”). Keywords: Comparative Genomic Hybridization; Genomic epidemiology; Gene-association study
