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Metatranscriptomics provides insight into the physiologies of the digestive tract symbionts within the medicinal leech, Hirudo verbana

ABSTRACT: Metatranscriptomics provides insight into the physiologies of the digestive tract symbionts within the medicinal leech, Hirudo verbana

OTHER RELATED OMICS DATASETS IN: GSE23786

PROVIDER: PRJNA130729 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR065788.fastq.gz	Fastqsanger.gz
	SRR065789.fastq.gz	Fastqsanger.gz

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Metatranscriptomics provides insight into the physiologies of the digestive tract symbionts within the medicinal leech, Hirudo verbana

Project description:Here, we report the use of Illumina RNA-Seq for investigating the physiology of the digestive-tract microbiome within the medicinal leech, Hirudo verbana. About 12 million cDNA reads were mapped against the genomes of the two dominant members of this simple microbiome. Results suggested that the most abundant, yet uncultured Rikenella-like bacterium forages host mucin glycans and ferments the carbohydrates to acetate that is secreted into the environment. The second dominant symbiont, Aeromonas veronii, appears to utilize the acetate secreted by Rikenella as a carbon and energy source, possibly linking the physiologies of the dominant symbionts. This study demonstrates how RNA-seq can be used to reveal the physiology of a naturally occurring microbiome.

2011-03-25 | GSE23786 | GEO

Hirudo verbana

Project description:Hirudo verbana overview

| PRJNA308876 | ENA

Hirudo verbana

Project description:Hirudo verbana Ganglion Transcriptome

| PRJNA656790 | ENA

Hirudo verbana

Project description:Hirudo verbana Raw sequence reads

| PRJNA504032 | ENA

Hirudo verbana

Project description:Hirudo verbana Central Nervous System Transcriptome

| PRJNA435743 | ENA

Hirudo verbana

Project description:Hirudo verbana Genome sequencing and assembly

| PRJNA294014 | ENA

Hirudo verbana

Project description:Hirudo verbana Genome sequencing and assembly

| PRJNA308798 | ENA

Hirudo verbana

Project description:Hirudo verbana Genome and Transcriptome sequencing and assembly

| PRJNA300575 | ENA

Hirudo verbana bloodmeal

Project description:Hirudo verbana bloodmeal raw amplicon sequencing reads

| PRJNA608698 | ENA

Characterization of a monoacylglycerol lipase in the medicinal leech, Hirudo verbana.

Project description:Endocannabinoids are a class of lipid neuromodulators found throughout the animal kingdom. Among the endocannabinoids, 2-arachydonoyl glycerol (2-AG) is the most prevalent endocannabinoid and monoacylglycerol lipase (MAGL) is a serine hydrolase primarily responsible for metabolizing 2-AG in mammals. In the medicinal leech, Hirudo verbana, 2-AG has been found to be an important and multi-functional modulator of synaptic transmission and behavior. However, very little is known about the molecular components of its synthesis and degradation. In this study we have identified cDNA in Hirudo that encodes a putative MAGL (HirMAGL). The encoded protein exhibits considerable sequence and structural conservation with mammalian forms of MAGL, especially in the catalytic triad that mediates 2-AG metabolism. Additionally, HirMAGL transcripts are detected in the Hirudo central nervous system. When expressed in HEK 293 cells HirMAGL segregates to the plasma membrane as expected. It also exhibits serine hydrolase activity that is blocked when a critical active site residue is mutated. HirMAGL also demonstrates the capacity to metabolize 2-AG and this capacity is also prevented when the active site is mutated. Finally, HirMAGL activity is inhibited by JZL184 and MJN110, specific inhibitors of mammalian MAGL. To our knowledge these findings represent the first characterization of an invertebrate form of MAGL and show that HirMAGL exhibits many of the same properties as mammalian MAGL's that are responsible for 2-AG metabolism.

| S-EPMC7245046 | biostudies-literature

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