Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1308109 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:This study examines whether maternal low ω6:ω3 ratio diet and offspring seaweed (SW) supplementation can improve offspring immunity and performance by elucidating the effects on piglet serum proteome. A total of 16 sows were given either a standard (CR, 13:1) or low ω6:ω3 ratio diet (LR, 4:1) during pregnancy and lactation and their male weaned piglets were supplemented with SW powder (4 g/kg, SW) or not (CT) in a 21-day post-weaning (PW) diet. Four PW piglet groups were then identified based on dam and piglet treatment, namely CRCT, CRSW, LRCT, and LRSW (n = 10 each). Piglet serum collected at weaning and d21 PW were analyzed (n = 5 each) using TMT-based quantitative proteomics and validated by appropriate assays.
2023-04-17 | PXD032327 | Pride
Project description:Characterisation of Bifidobacterium induced from prebiotic (galacto-oligosaccharide) supplemented pigs
| PRJNA766511 | ENA
Project description:Characterisation of Bifidobacterium induced from prebiotic (galacto-oligosaccharide) supplemented pigs
| PRJNA766512 | ENA
Project description:Characterisation of Bifidobacterium induced from prebiotic (galacto-oligosaccharide) supplemented pigs
| PRJNA767639 | ENA
Project description:probiotics and prebiotics to maternal diets is related to decreased incidence of diarrhea and greater weight gain during lactation. Our objective was to determine the impact of adding whole ground oat as a prebiotic alone or in combination with postbiotic yeast culture (YC) (Saccharomyces cerevisiae) to sow gestation and lactation rations on milk composition, piglet growth, and incidence of post weaning diarrhea (PWD). Diets: control (CON), CON + yeast culture (YC) [5g/kg], CON + oat (15% inclusion rate) (Oat) or CON+ YC [5g/kg] + Oat (15%) were fed during the last 30 days of gestation and throughout lactation (18-21 days). Shotgun proteome analysis of day 4 and 7 postpartum milk samples found 36 differentially abundant proteins (P-adj <0.1) in both Oat and YC supplemented sows relative to CON. Notable was increased expression of antimicrobial proteins, lactoferrin and chitinase. IgG in milk of Oat supplemented sows was lower than YC supplemented sows (p<0.05) but had greater E. coli-antigen reactivity. Piglet weights at birth were similar. At weaning YC + Oat piglets weighed less and gained less weight (p<0.05) postweaning than CON. The incidence of PWD was lowest in the YC and Oat groups compared to CON and YC+ Oat groups. These data suggest that Oat or YC culture supplementation alters milk immune and antimicrobial associated proteins that can impact piglets but may have negative effects on piglet growth when given in combination.
2020-12-05 | MSV000086562 | MassIVE
Project description:Early-life nutritional interventions are pivotal for promoting piglet growth and health, particularly in reducing weaning-associated disorders. This study investigated the effects of early-life Lactobacillus reuteri (L.reuteri) supplementation on growth performance, immune function, intestinal morphology, gut microbiota, ileal metabolites, and barrier function in suckling piglets. Neonatal piglets were administered L.reuteri for either 3 or 7 days post-birth. Results indicated that L.reuteri supplementation significantly increased weaning weight and average daily gain (ADG) while markedly reducing diarrhea incidence. Immune and antioxidant capacities were enhanced, evidenced by elevated serum and ileal IgG, IL-4, IL-10, T-SOD, T-AOC, and GSH-Px levels, alongside decreased pro-inflammatory cytokines (IL-1β, IL-6, TNF-α) and MDA. Histological analysis revealed improved intestinal architecture, characterized by increased ileal villus height and reduced crypt depth. 16S rRNA sequencing and metabolomic analyses showed that L.reuteri reshaped the gut microbiota by expanding beneficial Lactobacillus species and suppressing potential pathogens (Streptococcus, Pasteurellaceae), while modulating ileal metabolites involved in amino acid and energy metabolism. Multi-omics integration highlighted coordinated interactions between microbial composition and metabolites linked to improved health outcomes. Furthermore, the expression of tight junction proteins (Occludin, Claudin-3, ZO-1) and mucins (MUC-1, MUC-2) was significantly upregulated, indicating strengthened intestinal barrier integrity. Collectively, these findings demonstrate that early-life intervention with L.reuteri confers comprehensive benefits on suckling piglet health through immune enhancement, antioxidant protection, microbiota remodeling, metabolic regulation, and barrier reinforcement, supporting its potential as a practical strategy to improve early-life resilience and mitigate weaning-associated disorders in swine production.
2026-03-27 | MTBLS14164 | MetaboLights
Project description:It is increasingly recognised that the gastrointestinal microbiota plays a critical role in human health and promising evidence is accumulating that with dietary strategies, of prebiotic intervention, microbiota imbalances can be corrected and host health improved. Several prebiotics are widely used commercially in foods including inulin, fructo-oligosaccharides, galacto-oligosaccharides and resistant starches and there is convincing evidence, in particular for galacto-oligosaccharides, that prebiotics can modulate the microbiota and promote the growth of bifidobacteria in the intestinal tract of infants and adults. In this study we describe the identification and functional characterisation of the genetic loci responsible for the transport and metabolism of purified galacto-oligosaccharides (PGOS) by our model bifidobacterial strain, B. breve UCC2003. We further demonstrate that the extracellular endogalactanase specified by several B. breve strains, including B. breve UCC2003, is essential for metabolism of PGOS components with a long retention time and high degree of polymerisation. These PGOS components are transported into the bifidobacterial cell via various ABC transport systems and sugar permeases where they are further metabolised to galactose and glucose monomers that feed into the bifid shunt. This research described here advances our understanding of GOS metabolism by bifidobacteria and for the future there is great potential for exploiting bifidobacterial beta-galactosidase to create targeted prebiotics that can enrich for selected Bifiobacteria sp. and other beneficial microbes among the gut microbiota.
2014-12-31 | GSE37214 | GEO