Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A light-responsive monocyte subset reprogrammed by danger signals to suppress pathological pain

ABSTRACT: A light-responsive monocyte subset reprogrammed by danger signals to suppress pathological pain

PROVIDER: PRJNA1308965 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Danger signals activate the innate immune system of a filamentous fungus during regeneration

Project description:The ability to respond to injury is a biological process conserved across kingdoms, which in some cases results in regeneration of an organism’s lost part or structure. Due to their immobility, multicellular fungi are prey to a variety of predators and are therefore constantly exposed to mechanical damage. Nevertheless, our current knowledge of how fungi respond to injury is scarce. Activation of injury responses and regeneration relies on the detection of danger or alarm signals known as Danger- or Damage-Associated Molecular Patterns. We show that Ca2+ influxes and extracellular ATP, two such signals, together with Mitogen-Activated Protein Kinases, play a key role in the control of hyphal regeneration in the filamentous fungus Trichoderma atroviride. We further show that these signaling cascades activate major transcriptional changes early after injury. We identified a set of regeneration genes related to cell signaling, stress responses, regulation of transcription, ribosome biogenesis/translation, replication and DNA repair. We uncovered the activation of an innate immune response, including HET domain genes, until now only known to participate in vegetative incompatibility. We show that fungal and animal regeneration share danger-signals, signaling cascades, and the activation of an immune system, challenging the current notion of the evolution of this process.

2018-08-14 | GSE115811 | GEO

Changes of gene expression in DRG tissue after pathologic pain

Project description:To identify common mechanisms and targets regulating different types of pathological pain, we established models for neuropathic pain induced by spinal nerve ligation (SNL), inflammatory pain induced by complete Freund's adjuvant (CFA), chemotherapy pain induced by paclitaxel (PTX), and bone cancer pain induced by Lewis lung carcinoma (LLC) cells. We then collected dorsal root ganglion (DRG) tissues from mice for RNA-seq analysis.

2025-06-25 | GSE283287 | GEO

Genomics of Pain - Resilience to Pain

Project description:Genomics of Pain - Resilience to Pain

| PRJNA498368 | ENA

Genomics of Pain - Resilience to Pain

Project description:Genomics of Pain - Resilience to Pain

| PRJNA498369 | ENA

Danger signals activate the innate immune system of a filamentous fungus during regeneration

Project description:Danger signals activate the innate immune system of a filamentous fungus during regeneration

| PRJNA476116 | ENA

A novel subtype of astrocytes critical for HIV-associated pain pathogenesis

Project description:Pathological pain is a frequent complication in HIV patients, yet the underlying mechanism remains elusive, and effective therapeutic targets have not been identified. Reactive astrocytes are specifically activated in the spinal dorsal horn (SDH) of HIV patients with pathological pain and required for HIV-associated pain development in mouse models. These findings suggest that reactive astrocytes play a crucial role in the pathogenesis of HIV-associated pain. However, due to the heterogeneity of reactive astrocytes, the pathogenic subtype remains unknown. In this study, we used single-nucleus RNA-seq (snRNA-seq) transcriptomic analysis to identify a novel subtype of HIV-pain-associated astrocytes (HIPAs) in the lumbar spinal cord of HIV-1 gp120 transgenic models and in the spinal cord of HIV patients. HIPAs express galectin 3 (Gal3) and exhibit transcriptomic signatures of phagocytosis and inflammation. We demonstrated that HIPAs phagocytose neuronal components and are associated with neuronal degeneration. We also found that knockout (KO) of Gal3 in gp120 transgenic mice reduced the number of HIPAs. Furthermore, Gal3 KO inhibited the expression of mechanical allodynia and HIV pain-related pathogenic processes, including neuronal degeneration and neuroinflammation, in gp120 transgenic mice. Our data collectively suggest that HIPAs are a novel Gal3-expressing astrocytic subtype that plays a critical role in gp120-induced pathogenesis in the spinal pain neural circuit. Targeting HIPAs may provide a potential therapeutic strategy for treating HIV-associated pain.

2025-10-27 | GSE228974 | GEO

Danger signal initiates differentiation of monocytes into osteopontin‑producing macrophages III

Project description:Purpose: We report the application of single-cell RNA sequencing for profiling the genexpression of different monocyte types in order to characterize monocytes that differentiate into macrophages under danger signal in comparison to conventional GM-CSF and M-CSF macrophages Methods: Monocytes freshly isolated from PBMCs were differentiated into GM-CSF, M-CSF, and danger-signal induced macrophages. Two million cells of each macrophage population were tagged accordingly with TotalSeq™‑B Hashtags. Single cell RNA sequencing was performed on all macrophage populations in order to characterize cells from each population. ScRNAseq analysis was performed with the R package Seurat on 16470 barcodes after filtering. Results: Cluster analysis reveals distinct separation of all three macrophage populations. Conclusion: The clear difference in overall gene expression profile suggests that macrophages exposed to danger signal differentiate into a novel macrophage type in contrast to conventional GM-CSF and M-CSF monocytes.

2022-01-11 | GSE180113 | GEO

Engineered exosomes reprogram Gli1+ cells in vivo to prevent calcification of vascular grafts and autologous pathological vessels

Project description:Engineered exosomes reprogrammed Gli1+ cells to promote SMC reconstruction and inhibit calcification in TEVBs and autologous pathological vessels.

2023-08-08 | GSE227179 | GEO

Danger signal initiates differentiation of monocytes into osteopontin‑producing macrophages

Project description:Analysis of gene expression of monocytes differentiating into macrophages in the presence of a danger signal. Kinetic analysis (1 day, 2 days, 4 days, 7 days) and analysis of lipopolysaccharide stimulated 7 day macrophages in comparison to unstimulated 7 day macrophages.

2022-01-11 | GSE180027 | GEO

A Transcriptomic Analysis of Neuropathic Pain in the Spinal Cord after Nerve Injury

Project description:Neuropathic pain is a troublesome pathological condition without suitable treatments. In this study, we performed RNA sequencing (RNA-seq) analysis to reveal transcriptomic profiles of spinal cord from chronic constriction injury (CCI) rats.

2022-04-05 | GSE175760 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data