Project description:Transcriptional profile of snails exposed to irradiated E. paraensei miricidia and four days later challenged with S. mansoni miricidia. Compared to snails exposed to only irradiated E. paraensei miricidia.
Project description:Transcriptional profile of BS-90 snails injected with a cocktail of four FREP3 specific 27-mer DSiRNA oligos and two hours later exposed to S. mansoni miricidia. Compared to BS-90 snails injected with a cocktail of three GFP specific DSiRNA oligos and two hours later exposed to S. mansoni miricidia. Experiments were done over the course of 49 days. Snails were collected (10each) at 2 and 4 dpe to S. mansoni for comparison.
Project description:Transcriptional profiles of snails sized 12-20mm exposed to E. paraensei and unexposed controls Keywords: Dose response Five individual snails from the treatment group were analyzed at 12hours, 1, 2, 4, 8, and 16 days. Five unexposed control snails were also analyzed.
Project description:Transcriptional profile of snails with induced acquired resistance (exposed first to irradiated E. paraensei miricidia) after secondary challenge with viable miricidia. Compared to snails exposed to irradiated miricidia only or to viable miricidia only. Experiments were done over the course of 16 days, with 8 day intervals between sensitization and secondary challenge. Keywords: Dose response
Project description:In order to understanding the mechanisms of action of niclosamide, the active ingredient in the molluscicides, we used a Biomphalaria glabrata oligonucleotide microarray to investigate the effect of three sublethal exposures to niclosamide on the transcriptional responses of the snail B. glabrata relative to untreated snails. In addition to inducing biotransformation activity and inducing stress responses, niclosamide exposures also resulted in altered function of vesicle trafficking and may affect the snail’s oxygen binding capacity. In order to reveal how sublethal exposures of niclosamide to the snail B. glabrata affects the snail’s gene expression. The three sublethal doses of niclosamide tested are 0.15 mg/L (dose 1), 0.10 mg/L (dose 2), and 0.05 mg/L (dose 3). The control snails did not exposed to the niclosamid (0%). The snails were exposed to a given dose for 24 hours. Twenty live snails from each dose or control group were collected and randomly divided into four replicate groups, each with five snails, for subsequent RNA extraction and transcriptome analysis
Project description:Transcriptional profiles ofBS-90 snails sized 8-12mm exposed to E. paraensei (BS-90 are susceptible) and S. mansoni (BS-90 are resistant) and unexposed controls Keywords: Dose response Five individual snails from the treatment group were pooled to make up 3 repliactes. After exposure to the indicated parasite snails were collected and RNA was isolated at 12hours, 1, 2, 4, 8, 16, and 32 days. Three unexposed control groups were also analyzed.
