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BMP4 and BMP6 stimulation of adult mouse muscle stem cells activates the Notch signaling pathway

ABSTRACT: BMP4 and BMP6 stimulation of adult mouse muscle stem cells activates the Notch signaling pathway

PROVIDER: PRJNA1310614 | ENA |

REPOSITORIES: ENA

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BMP4 and BMP6 stimulation of adult mouse muscle stem cells activates the Notch signaling pathway [array]

Project description:Muscle stem cell (MuSC) activation, proliferation, self-renewal, and differentiation are tightly regulated processes that enable muscle development and regeneration without exhaustion of the stem cell pool. BMP signaling keeps MuSCs in a proliferative state and prevents their differentiation. We investigated the effect of bone morphogenetic protein 4 and 6 (BMP4 and BMP6) on transcriptional regulation and intracellular signaling of adult murine MuSCs using RNA-sequencing, RNA expression arrays, pathway-specific qPCR arrays, and Cleavage Under Targets and Tagmentation (CUT&Tag), which identifies the binding sites of transcription factors and the presence of specific histone marks with high spatial resolution. Freshly isolated MuSCs were grown for 42 to 72 hours, kept serum deprived for 6 hours, and then stimulated with BMP4/6. BMP4/6 addition resulted in a rapid increase in Inhibitor of DNA binding 1 (Id1)-expression, a typical BMP target gene, which peaked after one hour. As expected, stimulation with BMP4/6 resulted in a significant increase in transcription of BMP target genes such as Smad7, Nog, Id1, Klf10, and Lif, but also a strong increase in transcription of Notch pathway genes such as Hes1, Lfng, and Hey1. We showed that the important BMP pathway effectors SMAD4 and pSMAD1/5/9 bind to the promoters of these Notch targets. Our results suggest that the rapid expression of genes encoding Notch signaling components is due to direct regulation by BMP signaling.

2023-10-01 | GSE127798 | GEO

BMP4 and BMP6 stimulation of adult mouse muscle stem cells activates the Notch signaling pathway [array]

Project description:BMP4 and BMP6 stimulation of adult mouse muscle stem cells activates the Notch signaling pathway [array]

| PRJNA525422 | ENA

BMP4 and BMP6 stimulation of adult mouse muscle stem cells activates the Notch signaling pathway [CUT&Tag]

2025-09-17 | GSE305745 | GEO

BMP4 and BMP6 stimulation of adult mouse muscle stem cells activates the Notch signaling pathway [RNA-Seq II]

2025-09-17 | GSE305746 | GEO

BMP4 and BMP6 stimulation of adult mouse muscle stem cells activates the Notch signaling pathway [RNA-seq I]

2023-10-01 | GSE159594 | GEO

BMP4 and BMP6 stimulation of adult mouse muscle stem cells activates the Notch signaling pathway [CUT&Tag]

Project description:BMP4 and BMP6 stimulation of adult mouse muscle stem cells activates the Notch signaling pathway [CUT&Tag]

| PRJNA1307544 | ENA

BMP4 and BMP6 stimulation of adult mouse muscle stem cells activates the Notch signaling pathway [RNA-seq I]

Project description:BMP4 and BMP6 stimulation of adult mouse muscle stem cells activates the Notch signaling pathway [RNA-seq I]

| PRJNA669835 | ENA

BMP4 and BMP6 stimulation of adult mouse muscle stem cells activates the Notch signaling pathway [RNA-Seq II]

Project description:BMP4 and BMP6 stimulation of adult mouse muscle stem cells activates the Notch signaling pathway [RNA-Seq II]

| PRJNA1307542 | ENA

BMP4 and BMP6 stimulation of adult mouse muscle stem cells activates the Notch signaling pathway

Project description:This SuperSeries is composed of the SubSeries listed below.

2025-08-25 | GSE306430 | GEO

Transcription profiling of human mesenchymal stem cells treated for 8 hours with BMP6

Project description:We have established that BMP6 is an important endogenous regulator of human osteoblast differentiation. Our preliminary experiment showed that 8 hour BMP6 treatment induced early osteoblast markers in hMSC. In this study, we used microarrays to profile the global gene expression program in hMSC induced by BMP6 treatment and further identify the early osteogenic responses to BMP6 stimulation. Experiment Overall Design: The dataset contains a total of 4 gene chip measurements from duplicate experiments each with paired measurements of human MSC with or without 8 hours BMP6 treatment.

2009-06-08 | E-GEOD-13604 | biostudies-arrayexpress

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