Project description:Kidney proteomes of Rufous-collared sparrows from two different areas of the Atacama desert (Chile) were identified and compared by bottom-up proteomics utilizing nanoUPLC coupled online to tandem mass spectrometry. All experiments and sample collections were performed by the team of Dr. Paulina Gonzalez-Gomez. Proteomics sample preparation and biological mass spectrometry were performed in the laboratory of Prof. Dietmar Kueltz at UC Davis.
Project description:Regulatory small RNAs (sRNAs) represent a major class of regulatory molecules that play large-scale and essential roles in many cellular processes across all domains of life. Microbial sRNAs have been primarily investigated in a few model organisms and little is known about the dynamics of sRNA synthesis in natural environments, and the roles of these short transcripts at the community level. Analyzing the metatranscriptome of a model extremophilic community inhabiting halite nodules (salt rocks) from the Atacama Desert, sampled over two years with different weather conditions, with SnapT – a new sRNA annotation pipeline – we discovered hundreds of intergenic (itsRNAs) and antisense (asRNAs) sRNAs expressed.
