Project description:Tinospora Cordifolia, an ayurvedic herbal compound, is studied to be an effective drug in Autoimmine disease. Several in vivo mouse model systems are reported to show its efficiency as a drug of therapeutic potential to treat autoimmune diseases. Th17 cells are the major T cells involved in the inflammatory responses in autoimmune diseases. The mechanistic insight into the therapeutic efficiency of Tinospora cordifolia is yet not studied. In this study we have tried to unravel the mechanism of action for the effect of water extract of Tinospora cordifolia on Th17 cells using genome wide expression approach.
Project description:The Chinese sturgeon (Acipenser sinensis) is anadromous fish distributed in Yangtze River and East China Sea. In this study, we reported cleft-palate Chinese sturgeons in artificial population for the first time. In order to explore the genetic basis of palate malformation in A. sinensis, Illumina RNA-seq technology was used to analyze the transcriptome data of normal and cleft-palate individuals in farmed Chinese sturgeons. Raw reads were obtained and assembled into 808,612 unigenes, with an average length of 509.33 bp and an N50 of 574 bp. Sequence similarity analyses against four public databases (Nr, Uniprot, KEGG and COGs) found 158,642 unigenes that can be annotated. GABAergic synapse and TGF-β signal pathway were the most two enriched pathways with high Richfactor in the analyses of different expressed genes. In these two signal pathways, six genes (GABRA4, GS, GNS, S6K, PITX2, and BMP8) were found as cleft-palate genes in Chinese sturgeon. These findings contribute to our understanding of the genetic basis of cleft palate in sturgeon, while simultaneously adding to our knowledge about craniofacial development.
