Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1311520 | ENA |
REPOSITORIES: ENA
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Project description:1 day-, 14-day-, 8-week-, 8-month- and 12-month-old mice ovaries were collected for single-cell RNA sequencing. The developmental stage-specific somatic cells were defined and analyzed with the dynamics of cell-cell communication networks to reveal their association with ovarian development and senescence. These results provide strong support for further research on the supporting role and regulation mechanism of somatic cells to follicles.
2022-12-31 | GSE200612 | GEO
Project description:In this study, we selected differentially expressed miRNAs through construcing and analyzing the miRNA expression profile during 2-, 6-, and 12- month-old Small Tail Han Sheep ovaries, which provided a theoretical basis for the study of miRNAs regulating the reproduction of Small Tail Han Sheep. RNASeq techniques were used to perform profile analysis for these ovaries. The results showed that 11, 13 and 19 DE miRNAs were identified in 2- vs 6-, 6- vs 12-, and 2- vs 12-month-old ovaries, respectively. In total, 54, 37, and 198 predicted target genes of DE miRNAs were obtained from these three groups, respectively. GO and KEGG analyses showed that, in 2- vs 6-month-olds, the target genes of DE known sheep miRNAs were involved in 102 GO terms and 7 signaling pathways; in 6- vs 12-month-olds, the target genes of DE known sheep miRNAs were involved in 52 GO terms and 3 signaling pathways; and in 2- vs 12-month-olds, the target genes of DE known sheep miRNAs were involved in 88 GO terms and 6 signaling pathways. Three miR–target regulatory networks were constructed based on these DE miRNA–targets. 9 miRNAs were selected to validate the accuracy of miRNA sequencing data by qRT-PCR. The binding sites of oar-miR-432 with RPS6KA1 was validated by a dual luciferase reporter gene detection system. This is the first integrative analysis of miRNA and mRNA expression profiles in Small Tail Han Sheep ovarian development. These data help elucidate the molecular regulatory mechanisms in sheep ovarian development and identify the biomarkers that influence reproductive performance of Small Tail Han Sheep ewe.
2018-11-16 | GSE122607 | GEO
Project description:Spatial transcriptomics of 3-month-old mouse ovaries
| PRJEB55507 | ENA
Project description:This batch of data is for three independent projects. Since in this three projects, the mouse strains are all CD1/ICR, the sex are both female, and the mouse ages are all two-month-old, three projects shared control data to save expenditure legitimately. But in our hand we couldn't segregate the raw data into two parts corresponding to two projects, so we uploads all raw data. However, we also provided researcher-analyzable excel files including the expression of all phosphorylated proteins. The concise description of these three projects are as follow: Project 1: We found that in Chronic Resistant Stress (CRS)-treated mice, the fertility and oocyte quality significantly decreased, and plenty of biochemical indexes significantly changed, so we guessed that the overall physical health of CRS mice was severely impacted. As one major strategy to investigate the mechanism how CRS impact the physical health, We chose TMT-labeled quantitative ovarian phosphoproteomics service from Hangzhou PTM Bio Co. We sent two repeats of two-month-old CRS CD1/ICR mouse ovaries and two repeats of control ovaries for characterization of differentially phosphorylated proteins. Each repeat contained about 60 ovaries from 30 female mice, about 300 mg. Project 2: We found that in High Salt (HS)-treated mice, the fertility and oocyte quality significantly decreased, and plenty of biochemical indexes significantly changed, so we guessed that the overall physical health of HS mice was severely impacted. As one major strategy to investigate the mechanism how HS impact the physical health, We chose TMT-labeled Quantitative ovarian phosphoproteomics service from Hangzhou PTM Bio Co. We sent two repeats of two-month-old HS CD1/ICR mouse ovaries and two repeats of control ovaries for characterization of differentially phosphorylated proteins. Each repeat contained about 60 ovaries from 30 female mice, about 300 mg. Project 3: We found that in arecoline (Are)-treated mice, the fertility and oocyte quality significantly decreased, and plenty of biochemical indexes significantly changed, so we guessed that the overall physical health of Are mice was severely impacted. As one major strategy to investigate the mechanism how Are impact the physical health, We chose TMT-labeled Quantitative ovarian phosphoproteomics service from Hangzhou PTM Bio Co. We sent two repeats of two-month-old Are CD1/ICR mouse ovaries and two repeats of control ovaries for characterization of differentially phosphorylated proteins. Each repeat contained about 60 ovaries from 30 female mice, about 300 mg. Two repeats of control samples and data were shared among project 1, 2 and 3. Gross protein levels were also examined and used to normalize the protein phosphorylation level.
2024-05-22 | PXD028932 | Pride
Project description:Expression profiles were analyzed of mouse testis in four age groups (3, 6, 12, 18 postnatal month) by total RNA sequencing.
2021-09-24 | GSE175633 | GEO
Project description:We constructed four small RNA libraries from whole body of females, males (except ovaries and testes) and ovaries, testes of B. dorsalis for deep sequencing. The data analysis revealed 314 known and 221 novel miRNAs from these libraries. 14 female-biased and 12 male-biased miRNAs that may be involved in sexual differentiation were found by comparing the miRNA expression profiles in the four libraries.
2017-02-14 | GSE80536 | GEO
Project description:We found that in Chronic Resistant Stress (CRS)-treated mice, the fertility and oocyte quality significantly decreased, and plenty of biochemical indexes significantly changed, so we guessed that the overall physical health of CRS mice was severely impacted. As one major strategy to investigate the mechanism how CRS impact the physical health, We chose label-free quantitative ovarian crotonylproteomics service from Hangzhou PTM Bio Co. We sent two repeats of two-month-old CRS CD1/ICR mouse ovaries and two repeats of control ovaries for characterization of differentially crotonylated proteins. Each repeat contained about 60 ovaries from 30 female mice, about 300 mg. Gross protein levels were also examined and used to normalize the protein crotonylation level.
2024-05-22 | PXD028961 | Pride
Project description:In this study, we investigated the effects of Runx1 loss on ovarian pathogenesis in mice. Conditional loss of Runx1 in the somatic cells of the ovary led to an increased prevalence of ovarian tumors in aged mice. While ovaries from4.5 months old mice did not display tumors, they all contained abnormal follicle-like lesions. RNA-Sequencing analysis on 4.5 month old ovaries identified differentially expressed genes in Runx1 KO ovaries, including genes involved in metaplasia, ovarian cancer, epithelial cell development, tight junctions, cell-cell adhesion, and Wnt/beta-catenin pathway.
2023-08-08 | GSE211241 | GEO
Project description:12-month-old ATM-null mouse cerebellar transcriptome
| PRJNA1010812 | ENA