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ABSTRACT:
PROVIDER: PRJNA1312241 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Physarum polycephalum transcript analysis
| PRJNA79513 | ENA
Project description:Physarum polycephalum reference transcriptome
| PRJNA295269 | ENA
Project description:De novo RNA-Seq analytisis of plasmodia and sclerotia from Physarum polycephalum.
| PRJDB11632 | ENA
Project description:In multicellular organisms, the specification, coordination, and compartmentalization of cell types enable the formation of complex body plans. However, some eukaryotic protists such as slime molds generate diverse and complex structures while remaining in a multinucleated syncytial state. It is unknown if different regions of these giant syncytial cells have distinct transcriptional responses to environmental encounters, and if nuclei within the cell diversify into heterogeneous states. Here we performed spatial transcriptome analysis of the slime mold Physarum polycephalum in the plasmodium state under different environmental conditions, and used single-nucleus RNA-sequencing to dissect gene expression heterogeneity among nuclei. Our data identifies transcriptome regionality in the organism that associates with proliferation, syncytial substructures, and localized environmental conditions. Further, we find that nuclei are heterogenous in their transcriptional profile, and may process local signals within the plasmodium to coordinate cell growth, metabolism, and reproduction. To understand how nuclei variation within the syncytium compares to heterogeneity in single-nucleated cells, we analyzed states in single Physarum amoebal cells. We observed amoebal cell states at different stages of mitosis and meiosis, and identified cytokinetic features that are specific to nuclei divisions within the syncytium. Notably, we do not find evidence for predefined transcriptomic states in the amoebae that are observed in the syncytium. Our data shows that a single-celled slime mold can control its gene expression in a region-specific manner while lacking cellular compartmentalization, and suggests that nuclei are mobile processors facilitating local specialized functions. More broadly, slime molds offer the extraordinary opportunity to explore how organisms can evolve regulatory mechanisms to divide labor, specialize, balance competition with cooperation, and perform other foundational principles that govern the logic of life.
2022-03-02 | GSE173809 | GEO
Project description:We report a novel approach for studying the transcriptomic changes during cell differentiation, using Physarum as a model
2011-12-01 | GSE33793 | GEO
Project description:RNA-seq characterization of Physarum macroplasmodia
| PRJNA148107 | ENA
Project description:Complete characterization of the edited transcriptome of the mitochondrion of Physarum polycephalum using deep sequencing of RNA
| PRJNA79923 | ENA
Project description:We report a novel approach for studying the transcriptomic changes during cell differentiation, using Physarum as a model 3 samples examined: starved plasmodium, starved plasmodium collected 2 hours after photoinduction, and starved plasmodium collected 6 hours after induction
2011-12-01 | E-GEOD-33793 | biostudies-arrayexpress