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ABSTRACT:
PROVIDER: PRJNA1312490 | ENA |
REPOSITORIES: ENA
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Project description:Fescue toxicosis is a disease of wild and domestic animals grazing on fescue pasture infected with the endophytic fungus, Neotyphodium Coenophialum. Mice, previously selected for increased sensitivity to endophyte-infected fescue seed diets indicated by slow weight gain, were used to study the effects of fescue toxicosis on hepatic gene expression. Liver genes differentially expressed due to fescue toxins were studied using DNA microarray. A two-stage ANOVA of microarray data identified forty differentially expressed genes between mice fed endophyte-infected (E+) and endophyte-free (E-) fescue seeds. Significant Analysis of Microarray (SAM) analysis identified 9 genes as differentially expressed between treatment groups. Hierarchical clustering with the 40 genes identified by ANOVA clearly separate the mice according to their diets, with 100% confidence as computed by bootstrap analysis. Expressions of eleven genes were verified using quantitative real-time PCR (qPCR). The E+ diet resulted in downregulation of genes involved in sex-steroid metabolism pathway, genes involved in cholesterol and lipid metabolism. Keywords = Endophyte Keywords = Fescue Toxicosis Keywords = microarray Keywords: repeat sample
2005-01-26 | GSE2134 | GEO
Project description:The objectives of our study were to identify microRNA (miRNA) present in bovine sperm and to evaluate the effects of fescue toxicosis on sperm miRNA expression.
2014-12-10 | GSE61745 | GEO
Project description:Fecal microbiome from angus cattle experiencing fescue toxicosis
| PRJNA476327 | ENA
Project description:The objective of our study was to assess the effect of fescue toxicosis on the overall transcriptomics profile of liver tissue on growing Angus × Simmental steers and heifers. The susceptibility was determined by the T-snip genetic tests for a total of 42 pregnant cows. At mid-gestation, these animals were randomly assigned to control group which received a based diet and fed endophyte free fescue seeds; a treatment group was fed endophyte-infected tall fescue seeds for 30 days. RNA-seq experiments were performed in liver biopsy samples, and a total of 828 differentially expressed genes were detected.
2023-08-11 | GSE208241 | GEO
Project description:The objectives of our study were to identify microRNA (miRNA) present in bovine sperm and to evaluate the effects of fescue toxicosis on sperm miRNA expression. Angus bulls were assigned to treatments of either toxic or non-toxic fescue seed diets. Semen was collected and subjected to microRNA (miRNA) isolation after 126 days. Three bull's sperm miRNA samples from each treatment group were chosen and pooled for deep sequencing. Sequencing results were used to create a custom microarray for miRNA comparison between groups. LC Sciences was used as a service provider for the sequencing and custom microarray.
2014-12-10 | E-GEOD-61745 | biostudies-arrayexpress
Project description:Fescue toxicosis affects wild and domestic animals consuming ergot alkaloids contained in tall fescue forage infected with the endophytic fungus, Neotyphodium coenophialum. , When animals are consuming infected fescue forage during periods of elevated ambient temperatures (summer), a range of phenotypic disorders collectively called summer slump is observed. It is characterized by hyperthermia, with an accompanying decrease in feed intake, growth, milk yield and reproductive fitness. Laboratory mice also exhibit symptoms of fescue toxicosis a thermoneutral temperature, as indicated by reduced growth rate and reproductive fitness. Our goal was to characterize the differences in gene expression in liver of mice exposed to summer-type heat stress (HS) and infected fescue (E+) when compared to mice fed infected fescue at thermoneutral temperature (TN). Mice were fed E+ diet under HS (34 ± 1°C; n = 13; E+HS) or thermoneutral (TN) conditions (24 ± 1°C; n = 14; E+TN) for a period of two weeks between 47 to 60 d of age. Genes differentially expressed between E+HS versus E+TN were identified using DNA microarrays. Forty-one genes were differentially expressed between treatment groups. Expressions of eight genes were measured using quantitative real-time PCR. Genes coding for phase I detoxification enzymes were up-regulated in E+HS mouse liver. This detoxification pathway is known to produce reactive oxidative species. We observed an up-regulation of genes involved in the protection against reactive oxidative species. Key genes involved in de novo lipogenesis and lipid transport were also up-regulated. Finally, genes involved in DNA damage control and unfolded protein responses were down-regulated. Keywords: Stress response
2006-08-29 | GSE5642 | GEO
Project description:Effects of fescue toxicosis on rat hepatic tissue
| PRJNA92665 | ENA
Project description:Metagenomic analysis revealed significant changes in cattle rectum microbiome and antimicrobial resistome under fescue toxicosis
| PRJNA744219 | ENA
Project description:Background: This study investigated the effects of dietary soy isoflavone supplementation on reproductive performance, antioxidant capacity, and colostrum-derived exosomal microRNA profiles in sows, with the aim of exploring the molecular basis of maternal–offspring integrated regulation. A total of 120 Landrace × Yorkshire sows were assigned to either a control diet or a diet supplemented with 200 mg/kg soy isoflavone from gestation day 106 to lactation day 28. Reproductive performance and serum antioxidant indices were evaluated, and colostrum-derived exosomes were isolated for small RNA sequencing and bioinformatic analysis. Results: Dietary soy isoflavone supplementation significantly increased the total number of piglets born, the number of live-born piglets, litter weight at birth, and litter weight at weaning, while shortening farrowing duration (P < 0.05). In addition, soy isoflavone significantly elevated serum total antioxidant capacity and catalase activity on lactation day 21 (P < 0.05). Transmission electron microscopy, nanoparticle tracking analysis, and flow cytometry confirmed the successful isolation of colostrum-derived exosomes. Small RNA sequencing showed that most microRNAs ranged from 18 to 26 nucleotides, with a predominant peak at 22–23 nucleotides. Principal component analysis and differential expression analysis revealed that soy isoflavone markedly reshaped the microRNA cargo of colostrum-derived exosomes. Functional enrichment analysis indicated that the predicted target genes of differentially expressed microRNAs were mainly involved in transcriptional regulation, kinase-mediated signaling, inflammatory responses, and metabolic pathways, including mitogen-activated protein kinase, Ras, Rap1, endocytosis, autophagy, and adherens junction pathways. Integrated network analyses further suggested coordinated regulation of inflammatory signaling and metabolic homeostasis. Conclusions: Dietary soy isoflavone supplementation improved sow reproductive performance and antioxidant capacity, while reshaping colostrum-derived exosomal microRNA profiles and their associated regulatory networks. These findings provide a potential molecular basis for maternal–offspring integrated regulation.
2026-04-16 | GSE327773 | GEO