Fejervarya limnocharis
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1313576 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Fejervarya cancrivora Raw sequence reads
| PRJNA643354 | ENA
Project description:Fejervarya multistriata Raw sequence reads
| PRJNA831371 | ENA
Project description:Fejervarya multistriata Genome sequencing and assembly
| PRJNA553323 | ENA
Project description:Fejervarya cancrivora Transcriptome or Gene expression
| PRJNA258254 | ENA
Project description:Organisms adapt to environmental fluctuations by varying their morphology and structural, physiological, and biochemical characteristics. Gut microbiome, varying rapidly in response to environmental shifts, has been proposed as a strategy for adapting to the fluctuating environment (e.g., new dietary niches). Here, we explored the adaptive mechanism of frog intestinal microbes in response to environmental changes. We collected 170 Fejervarya limnocharis during different seasons (spring, summer, autumn, and pre-hibernation) to study the compositional and functional divergence of gut microbiota and analysed the effects of seasonal feeding habits and body condition on intestinal microorganisms using 16S rRNA high-throughput sequencing, Tax4Fun function prediction analysis, and bioinformatics analysis. The results showed no significant dietary difference in various seasons and between males and females. However, a significantly positive correlation was detected between dietary diversity and food niche width. Host condition (body size, body mass, and body condition) also revealed seasonal changes. The frogs were colonised by 71 bacterial phyla and dominated by Proteobacteria, Firmicutes, and Bacteroidetes. Stenotrophomonas was the most abundant genus in the Proteobacteria. The composition, diversity, and function of intestinal microorganisms in different seasons were significantly different. Significant differences were observed in composition and function but not in the microbial diversity between sexes. Furthermore, seasonal foods and body mass were significantly correlated with gut microbial composition. Our results suggest that gut microbiomes of F. limnocharis vary seasonally in response to diet under fluctuating environments.
| S-EPMC8153623 | biostudies-literature
Project description:Crab-eating frogs (Fejervarya cancrivora) can live in brackish water with a salinity of up to 18‱, although most amphibians are not able to tolerate such high saline environments. To investigate its potential osmoregulation, we conducted experiments in F. cancrivora and F. multistriata. The results showed that F. cancrivora made use of ions (such as Na+ and Cl-) to increase intracellular concentrations via the Na+/K+-ATPase (NKA) enzyme. The mRNA expression of aldose reductase (AR) was significantly higher in F. cancrivora (p < 0.05), indicating that more organic osmolytes were produced and transported to maintain cellular homeosis. The mRNA expressions of Aquaporin 1 (AQP1) and AQP3 in kidney were significantly higher in F. cancrivora, while AQP expression in skin was higher in F. multistriata (p < 0.05). The mRNA level in activating the transcription of the nuclear factor of activated T cells-5 (NFAT5) which is one of the target genes of regulating the cellular response to hypertonicity, was higher in F. cancrivora. The protein expression of CDK5, the upstream protein of the NFAT5 pathway, was 2 times higher in F. cancrivora. Therefore, we can conclude that CDK5/NFAT5-regulated transporters might be involved in osmoregulation in F. cancrivora.
| S-EPMC9220195 | biostudies-literature