Project description:We established NCI-H1703 cells with stable PPM1G knockdown using short hairpin RNA (shRNA) technology. Subsequently, we performed data acquisition and analysis of high-throughput RNA sequencing (RNA-seq) by comparing these PPM1G-knockdown cells with wild-type NCI-H1703 cells.
Project description:Comparing the effects of caffeine on cells expressing either WT 3HA-TOR1 or 3HA-TOR1 [I1954V + W2176R] Keywords: Dose response in wild type versus mutant
Project description:To explore transcriptomic mechanisms underlying the proliferative, dedifferentiated phenotypes observed in ARID1A knockdown MMNK1 cell, We performed RNA-seq analysis on MMNK1 cells infected with sh-ARID1A versus sh-Scramble lentiviruses
Project description:Genome wide mRNA and miRNA profiling was performed in SH-SY5Y cells stably overexpressing wild type or mutant MIR204 or MIR618. Mutants came from a large scale genetic screening of brain expressed miRNA genes in patients with schizophrenia or idiopathic generalized epilepsy and in control individuals. Based on enrichment of the variants with the schizophrenic or epileptic phenotype and based on impact prediction, two variants, one near MIR204 (rs7861254) and one in MIR618 (rs2682818) were selected for functional validation. Genome wide profiling of mRNA (micro-array) and mature miRNAs (small RNA sequencing, submitted to SRA) was performed in the created stable cells to assess the effect of the variants and to investigate the function of these miRNA genes. Micro-array analysis on HTA2.0 array was performed at AROS Applied Biotechnology, Denmark. SH-SY5Y cells (purchased from Sigma-Aldrich, catalog number: 94030304-1VL) were transduced with MIR204 or MIR618 wild type or mutant constructs. The stable cells were then split to obtain biological replicates and were cultured separately prior to total RNA extraction and micro-array analysis on the HTA2.0 array. For MIR204 analysis: 2 wt cells versus 3 mutant cells; for MIR618 analysis: 3 wt cells versus 3 mutant cells were used.
Project description:The Treatment Choices, Duration and Outcomes in Patients with Ras Wild Type (RAS WT) Metastatic Colorectal Cancer (mCRC). Retrospective, Multi-Center, Real-World Data Analysis
Project description:By comparing wild-type SK-N-SH neuroblastoma cells to cells over-expressing either wild-type or R252W mutant MORC2, the goal of the experiment was to assess the effect of MORC2 over-expression on the transcriptome.
Project description:camta3-1, camta3-2 and WT microarray experiments, statistical and bioinformatics analysis Keywords: expression analysis; mutant versus wild type comparison
