Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1314309 | ENA |
REPOSITORIES: ENA
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Project description:Recent studies have identified the pervasive presence of nano- and microplastics (NMP) in atmospheric air, leading to inevitable human exposure. Human skin, with an extensive surface area of 1.5-2 m², is constantly exposed to airborne NMP, yet the dermal implications of such exposure remain elusive. Our investigation employed fragmented polystyrene (fPS), a plastic commonly found in the air, to evaluate dermal penetration using various skin models. We utilized pyrolysis-gas chromatography/mass spectrometry (py-GC/MS) to quantify fPS that had penetrated the skin model. Furthermore, we examined the biological alterations in skin keratinocytes consequent to fPS exposure and analyzed the regulation of inflammatory response markers. Our findings reveal deleterious effects on skin keratinocytes, underlining the potential risks posed by short-term fPS exposure. This multifaceted skin model study offers new perspectives on the toxicological impacts of NMP.
2024-02-19 | GSE255709 | GEO
Project description:Escherichia coli of the B2 phylotype reside in human and animal intestines. We addressed the questions which host cell processes were dysregulated by E. coli HlyA that can potentiate intestinal diseases. The colon carcinoma cell line Caco-2 was infected by HlyA+ E. coli. Cell polarity regulation was analyzed by live cell imaging for the localization of phosphatidylinositol-4,5-bisphosphate (PIP2) abundance in the plasma membrane. In Caco-2 monolayers, transepithelial electrical resistance was measured to determine barrier function. Cell morphologyproliferation and dissemination was assessed microscopically. Cell signaling and polarity regulation was analyzed by RNA-Seq.
2021-09-01 | GSE169213 | GEO
Project description:The contamination of marine ecosystems with microplastics, such as the polymer polyethylene, a commonly used component of single-use packaging, is of global concern. Although it has been suggested that biodegradable polymers, such as polylactic acid, may be used to replace some polyethylene packaging, little is known about their effects on marine organisms. Blue mussels, Mytilus edulis, have become a “model organism” for investigating the effects of microplastics in marine ecosystems. We show here that repeated exposure, over a period of 52 days in an outdoor mesocosm setting, of M. edulis to polyethylene microplastics reduced the number of byssal threads produced and the attachment strength (tenacity) by ~50%. Exposure to either type of microplastic altered the haemolymph proteome and, although a conserved response to microplastic exposure was observed, overall polyethylene resulted in more changes to protein abundances than polylactic acid. Many of the proteins affected are involved in vital biological processes, such as immune- and stress- regulation, metabolism and cellular and structural development. Our study highlights the utility of mass spectrometry-based proteomics to assess the health of key marine organisms and identifies the potential mechanisms by which microplastics, both conventional and biodegradable, could affect their ability to form and maintain reefs.
2019-01-02 | PXD011567 | Pride
Project description:Microplastics are a relatively newly discovered environmental hazard that can contribute to the disruption of many physiological processes in the organism. There is evidence that they affect the physiology of the pancreas, but research is still very limited. Therefore, the aim of the study was to determine the effects of PET microplastics on the global proteomic profile of the porcine pancreas using LC-MS/MS analysis. The pigs were treated with a low (0.1 g/day) or a high dose (1 g/day) of PET microplastics for 4 weeks. The analysis revealed that PET microplastics affected protein expression in a dose-dependent manner - the low dose affected the abundance of 7 proteins, while the high dose of 17.
2025-07-07 | MSV000098442 | MassIVE
Project description:Effluents from sewage treatment plants contain a mixture of micropollutants with the potential of harming aquatic organisms. Thus, addition of advanced treatment techniques to complement existing conventional methods has been proposed. Some of the advanced techniques could, however, potentially produce additional compounds affecting exposed organisms by unknown modes of action. In the present study the aim was to improve our understanding of how exposure to different sewage effluents affects fish. This was achieved by explorative microarray and quantitative PCR analyses of hepatic gene expression, as well as relative organ sizes of rainbow trout exposed to different sewage effluents (conventionally treated, granular activated carbon, ozonation (5 or 15 mg/L), 5 mg/L ozone plus a moving bed biofilm reactor, or UV-light treatment in combination with hydrogen peroxide). Exposure to the conventionally treated effluent caused a significant increase in liver and heart somatic indexes, an effect removed by all other treatments. Genes connected to xenobiotic metabolism, including cytochrome p450 1A, were differentially expressed in the fish exposed to the conventionally treated effluents, though only effluent treatment with granular activated carbon or ozone at 15 mg/L completely removed this response. The mRNA expression of heat shock protein 70 kDa was induced in all three groups exposed to ozone-treated effluents, suggesting some form of added stress in these fish. The induction of estrogen-responsive genes in the fish exposed to the conventionally treated effluent was effectively reduced by all investigated advanced treatment technologies, although the moving bed biofilm reactor was least efficient. Taken together, granular activated carbon showed the highest potential of reducing responses in fish induced by exposure to sewage effluents.
2012-05-10 | GSE37574 | GEO
Project description:Global cellular biological processes modulated by Dasatinib treatment in renal cell carcinoma
| PRJNA321029 | ENA