Project description:Amblyomma variegatum, the tropical bont tick, is a species of veterinary importance being considered as one of major pest of ruminants in Africa and in the Caribbean. It is associated with severe dermatophilosis and transmits the rickettsiale Ehrlichia ruminantium, the agent of heartwater, to ruminants. Tick’s saliva contains a complex mixture of bioactive molecules including proteins that modulate host responses to ensure successful blood feeding. The limited amount of saliva that can be obtained from ticks has hampered characterisation of salivary proteins using traditional protein chemistry, but recent advances in proteomics (especially mass spectrometry and bioinformatics) provide new tools to finely investigate tick sialomes. The general objective of this study was to better understand the role of A. variegatum saliva in tick/host/pathogen interactions. Salivation of semi-fed females was induced after detachment by injection of pilocarpin. Crude saliva proteins were pre-fractioned by SDS-PAGE and trypsin-generated peptides were analysed online by nano-flow Ultimate 3000 HPLC–nanoelectrospray ionization using an Orbitrap Elite mass spectrometer. Raw data analysis was performed using the MaxQuant software and proteins identified according to UniProt Knowledgebase. Our results bring new insights for a better understanding of tick-ruminant interactions at the molecular level, and pave the way towards integrative strategies to interfere with both the immunomosuppressive and infectious processes in corresponding tick-borne diseases.
2018-01-05 | PXD007821 | Pride
Project description:Microbial diversity associated with the camel tick Hyalomma dromedarii
Project description:Ixodes species ticks are competent vectors of tick-borne viruses including tick-borne encephalitis and Powassan encephalitis. Tick saliva has been shown to facilitate and enhance viral infection. This likely occurs by saliva-mediated modulation of host responses into patterns favorable for viral infection and dissemination. Because of the rapid kinetics of tick-borne viral transmission, this modulation must occur as early as tick attachment and initiation of feeding. In this study, the gene expression profile of cutaneous bite-site lesions created by uninfected ticks were analyzed at 1, 3, 6, and 12 hours after Ixodes scapularis nymphal tick attachment to discover host pathways or responses potentially important in tick-borne viral establishment.