Similar Datasets
Project description:Repo-Man chromatin binding sites were obtained by expression of GST:Repo-Man and incubation with nucleosomes extracted from HeLa cells (GST alone signal was used as a negative control and subtracted from the GST:Repo-Man chromatin bound fraction)
2016-11-23 | GSE84035 | GEO
Project description:Expression profile in Overexpression of GST,GST-Med3
| PRJNA274807 | ENA
Project description:To more specifically define the sequence content that favors direct binding of GAF, a genomic-context protein binding microarray (gcPBM) was used to detect GST-tagged GAF binding to double stranded DNA probes using a fluorescently conjugated anti-GST secondary antibody
2018-02-15 | GSE110653 | GEO
Project description:Goal: Identification of RNAs that are associated with the novel Ferry complex Methods: We performed a GST pulldown experiment, where GST-Ferry and GST were incubated with a HEK 293 cell lysate and extensively washed prior to elution. Subsequently, RNA was extracted from the elution and sequenced. Results: We identifed around 17 588 different transcripts and found a set of 252 mRNAs that are specifically enriched in the GST-Ferry sample. Conclusions: The Ferry complex seems to interact with a specific subset of mRNAs
2023-04-27 | GSE173422 | GEO
Project description:We have used microarrays to study the gene expression changes in Overexpression of GST,GST-Med3 of yeast cells to detect how the overexpressioin may affect global gene expression.
2015-08-06 | GSE65666 | GEO
Project description:The TET3 CXXC domain has unique DNA binding properties. It binds to DNA in a cytosine-dependent manner that prefers binding to CpG dinucleotides but is not restricted by the CpG-content, distinct from other well-characterized CXXC domains. To map the TET3 CXXC domain binding sites across the human genome, we purified the GST-tagged TET3 CXXC domain protein and performed the GST pull-down assay using the genomic DNA purified from HEK293T cells. The enriched DNA fragments were then sequenced and aligned to human genome(hg19). We used the GST pull-down assay followed by DNA deep sequencing to map the DNA bound by the TET3 CXXC domain in vitro.
2012-12-10 | E-GEOD-41551 | biostudies-arrayexpress
Project description:We have used microarrays to study the gene expression changes in Overexpression of GST,GST-Med3 of yeast cells to detect how the overexpressioin may affect global gene expression. RNA samples were taken from yeast cells overexpressed GST or GST-med3 and hybridized to affymetrix microarrays: GST and GST-Med3
2015-08-06 | E-GEOD-65666 | biostudies-arrayexpress
Project description:As global life expectancy continues to increase, shifting our focus from solely extending lifespans to actively pursuing healthy ageing is crucial. GSTA1-3 members of the alpha class of glutathione S-transferase are involved in diverse biological processes, including metabolism and immune regulation, indicating their influence on human health and lifespan. We used Caenorhabditis elegans as a model organism to examine the impact of gst-35, an orthologous gene of mammalian GSTA1, GSTA2, and GSTA3, on healthy ageing. Our findings indicated that gst-35 overexpression had deleterious effects on various physiological aspects of nematodes. The overexpression specifically caused a significant reduction in nematode lifespan, inhibited nematode development and growth, and substantially diminished reproduction, physical fitness, and stress resistance. Conversely, gst-35 knockout in nematodes showed a partial improvement in physical fitness and stress resistance. Comprehensive RNA-sequencing transcriptome analysis revealed that gst-35 overexpression perturbed metabolic homeostasis. Additionally, gst-35 overexpression induceds lysosomal dysfunction via pmk-1 and skr, affecting the process of healthy ageing. These findings unravel the intricate role of gst-35 in the ageing process, contributing to the expanding knowledge in the field of healthy ageing research.
2024-07-31 | GSE273339 | GEO