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HIF1A regulated genes in MCF10A cells after treatment with Oncostatin M or control ligands

ABSTRACT: HIF1A regulated genes in MCF10A cells after treatment with Oncostatin M or control ligands

PROVIDER: PRJNA1314784 | ENA |

REPOSITORIES: ENA

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HIF1A regulated genes in MCF10A cells after treatment with Oncostatin M or control ligands

Project description:We employed a single-cell RNA sequencing approach to study the role of Oncostatin M (OSM) in inducing collective cell migration in MCF10A cells and its upregulation of HIF1A. To identify HIF1A-dependent transcriptional programs, we stably knocked down HIF1A using shRNA and treated cells with OSM.

2025-09-08 | GSE307221 | GEO

Identifying transcripts regulated by p53 in MCF10A cells

Project description:Identifying transcripts regulated by p53 in MCF10A cells

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neonatal rat cardiomyocytes stimulated by Oncostatin M

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2015-02-27 | E-MTAB-2560 | biostudies-arrayexpress

KIMAT1 and HIF1A-As2 silencing RNA-seq

Project description:In our study, we valided the important oncogeneic roles of KIMAT1 and HIF1A-As2 in non-small cell lung cancer. We used custom designed GapmeRs to silence the KIMAT1 and HIF1A-As2 in H1299 and performed RNA-seq. 11389 and 7767 genes were regulated by KIMAT1 and HIF1A-As2, respectively.

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KIMAT1 and HIF1A-As2 silencing small RNA-seq

Project description:In our study, we valided the important oncogeneic roles of KIMAT1 and HIF1A-As2 in non-small cell lung cancer. We used custom designed GapmeRs to silence the KIMAT1 and HIF1A-As2 in H1299 and performed small RNA-seq. 214 and 233 microRNAs were regulated by KIMAT1 and HIF1A-As2, respectively.

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Exercise-induced Crosstalk between Immune Cells and Adipocytes in Humans: Role of Oncostatin-M

Project description:The discovery of novel exercise-regulated circulatory factors has fueled interest in the role of organ-crosstalk, especially between skeletal muscle and adipose tissue in mediating beneficial effects of exercise. We studied the adipose tissue transcriptome in men and women with normal glucose tolerance or type 2 diabetes following an acute exercise bout, revealing substantial exercise and time-dependent changes, and a sustained increase in inflammatory genes unique to Type 2 diabetes. We identify Oncostatin-M as one of the most upregulated adipose tissue secreted factors post exercise. In cultured human adipocytes, oncostatin-M enhanced MAPK signalling and regulates lipolysis. Oncostatin-M expression predominantly arises from adipose tissue immune cell fractions, while the corresponding receptors are expressed in adipocytes. Oncostatin-M expression is induced in cultured human Thp1 macrophages in response to exercise-like stimuli. Our results suggest immune cells, via secreted factors such as oncostatin-M, are important in crosstalk between skeletal muscle and adipose tissue during exercise, to regulate adipocyte metabolism and adaptation.

2023-11-21 | GSE198922 | GEO

Identifying transcripts regulated by p53 in MCF10A cells

Project description:Total RNA was extracted from five million wild type (WT) and p53 knockout (p53_KO) MCF10A cells using Qiagen RNAeasy kit. Ribosomal RNAs were removed and the rest of RNAs were subject to RNAseq.

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Identify novel TGF-β-regulated genes by RNA-sequencing from MCF10A cells

Project description:Identify novel TGF-β-regulated genes by RNA-sequencing from MCF10A cells

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Gene expression profiling in WT and Hif1a-/- HSCs

Project description:To understand the underlying mechanism by which the Hif1a gene is required by hematopoietic stem cells (HSCs), we performed a comparative DNA microarray analysis using total RNA isolated from wild type Lin-Sca-1+c-Kit+ cells and Hif1a-/- Lin-Sca-1+c-Kit+ cells. The result was validated by quantitative real-time PCR analysis of wild type Lin-Sca-1+c-Kit+ and Hif1a-/- Lin-Sca-1+c-Kit+ cells. We compared the gene profile of HSCs between WT mice and Hif1a-/- mice.

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Gene expression profiling in BCR/ABL-expressing LSCs and BCR/ABL-expressing Hif1a-/-LSCs

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