Project description:The main objective of the present proteomic study is to identify the metabolic response, in particular theglycan uptake and degradation machinery, conferring members of Roseburia growth on HMOs and/or onrelated O-glycans. Accordingly, the proteomes of R. hominis and R. inulinivorans bothgrown on humanmilk oligosaccharides (HMOs), were compared to glucose to reveal the molecular basis for growth onHMOs. Furthermore, we compare the proteomes of R. hominis and R. inulinivorans grownin co-culturewith A. muciniphilia either on mucin or on glucose to identify potential metabolic routes of mucin derivedO-glycan utilization in Roseburia.
Project description:The present study identified the role of the Mitochondrial Fission Process 1 protein (MTFP1) in the mitochondrial and metabolic activity of the liver. Ablation of Mtfp1 liver cells alters mitochondrial function and confers a specific liver metabolic protection against high-fat diet
Project description:Hesperidin, a citrus flavonoid glycoside, was investigated for its protective effects against high-fat diet (HFD)-induced metabolic syndrome in mice. Twelve weeks of supplementation markedly attenuated body weight gain, hepatic steatosis, adipocyte hypertrophy, dyslipidemia, and systemic inflammation, while enhancing glucose tolerance and insulin sensitivity. 16S rRNA sequencing demonstrated that hesperidin partially restored microbial diversity and selectively enriched beneficial taxa, including Lactobacillus, Bifidobacterium, and Akkermansia. Serum metabolomics revealed increased levels of microbial-derived metabolites such as cinnamic acid, hippuric acid, and sulfated phenolic acids, compounds associated with anti-obesity, antioxidant and anti-inflammatory activities. Transcriptomic profiling of inguinal white adipose tissue identified broad remodeling of metabolic pathways, with notable activation of calcium signaling, implicating both UCP1-dependent browning and UCP1-independent calcium futile cycling in thermogenesis. Importantly, antibiotic treatment abolished the metabolic benefits and suppressed the generation of bioactive metabolites, underscoring the indispensable role of gut microbiota in hesperidin bioactivity. Together, these findings delineate a microbiota–metabolite–adipose tissue axis through which hesperidin confers systemic metabolic protection, highlighting its potential as a microbiota-targeted dietary strategy for managing obesity-related disorders.
