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ABSTRACT:
PROVIDER: PRJNA1320961 | ENA |
REPOSITORIES: ENA
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Project description:Purpose: We aimed to use RNA-Seq and de novo transcriptome assembly to produce transcriptomes and analyze the changes in transcription regulation associated with cold and heat treatment in Spodoptera frugiperda. A detailed differential expression analysis revealed a number of candidate genes that may be associated with FAW cold and heat tolerance. To validate the RNA-seq results, we used qRT-PCR. We hoped to provide a foundation for the adaptive mechanism as well as a rich resource for the discovery and detection of novel genes involved in cold and heat stress responses of Spodoptera frugiperda. Methods: Spodoptera frugiperda was reared at lab condition, and incubated at 2 different temperature( 4 and 40°C) for 16 hours as temperature treatment groups. RNA was extracted using Trizol reagent and Illumina sequencing was performed at Macrogen. Illumina short reads were quality-filtered and Illumina-based de novo transcriptome assembly was performed. Differential Gene Expression Analysis was studied for different tempearture conditions. Results: A total of 211,967 unigenes were collected, at least 14,338 of which were annotated with gene descriptions, gene ontology terms, and metabolic pathways. There were 50 GO functional sub-groups and 21 EggNOG words as a result. DEGs with log2FC≥2 were identified and compared at various temperatures. In comparison to the 25°C treated group, we discovered 199 and 1248 individual DEGs co-regulated at 4 and 40°C, respectively. Comparing transcriptome profiles for differential gene expression revealed a number of DE genes, including cytochrome P450, odorant binding proteins (OBP), and immune system genes previously implicated in cold and high temperature resistance. The enrichment pathways were identified using KEGG analysis, and heatmaps of similar unigenes from both treatment groups (T4 and T40) were plotted. We used qPCR to confirm the RNA-seq data on 10 up- and down-regulated DEGs. Conclusions: In conclusion, we used RNA-Seq technology focused on high-throughput sequencing to compare the transcriptomes of Spodoptera frugiperda under high- and low-temperature stresses. This research was the first to identify a large number of genes that were significantly up-regulated at high and low temperatures. Many genes were discovered through comparative transcriptome analysis, and a significant number of improvements in metabolic pathways were discovered through GO and KEGG enrichment analysis. Our findings will help future molecular research and genomic studies. These newly found genes may be important and necessary to FAW harsh environment tolerance and its behavior for adaptation in new environment as well as quarantine area.
2021-05-26 | GSE175545 | GEO
Project description:We report the application of whole transcriptome sequencing technology for high-throughput profiling of coding and non-coding RNAs associated with Spodoptera frugiperda feeding in Zea mays. 4,366 mRNAs and 233 lncRNAs were differentially expressed during Spodoptera frugiperda feeding in Zea mays. Our data contribute to the understanding of the function of coding and non-coding RNAs in the regulation of plant-insect interactions.
2023-11-21 | GSE247976 | GEO
Project description:Insect gut microbiota plays important roles in acquiring nutrition, preventing pathogens infection, immune responses, and communicating with the environment. Gut microbiota can be affected by some external factors such as foods, temperature, and antibiotics. Spodoptera frugiperda (Lepidoptera: Noctuidae) is an important destructive pest of grain crops all over the world. The function of gut microbiota in S. frugiperda remains to be investigated. In this study, we fed the S. frugiperda with the antibiotic mixture (penicillin, gentamicin, rifampicin, and streptomycin) to perturb the gut microbiota, and further examined the effect of dysbiosis in gut microbiota on the gene expression of S. frugiperda by RNA sequencing. We found the composition and diversity of the gut bacterial community were changed in S. frugiperda after antibiotics treatmen, and the expression of genes related to energy and metabolic process were affected after antibiotics exposure in S. frugiperda. Our work will help understand the role of gut microbiota in insects.
2021-08-06 | GSE175476 | GEO
Project description:Small nucleolar RNAs (snoRNA) function in guiding 2'-O-methylation and pseudouridylation of ribosomal RNAs. But we found that knock down of a C/D box snoRNA, Bm-15, can induce apoptosis of insect Spodoptera frugiperda Sf9 cells. For the genome sequence of Spodoptera frugiperda is incomplete, here with the de novo sequencing method, transcriptome of Spodoptera frugiperda cell line Sf9 were sequenced after being transfected with overexpression vector and repression probes of snoRNA Bm-15. Results showed that 21 apoptosis-related genes were up-regulated upon Bm-15 inhibition and down-regulated with Bm-15 overexpression.
2019-04-17 | GSE98196 | GEO
Project description:This SuperSeries is composed of the following subset Series: GSE16775: Effect of HdIV or MdBV injection on the Spodoptera frugiperda hemocyte transcriptome GSE16776: Effect of HdIV or MdBV injection on the Spodoptera frugiperda fat body transcriptome Refer to individual Series
2011-04-05 | E-GEOD-17293 | biostudies-arrayexpress
Project description:Comparison of the effects of 2 polydnaviruses (HdIV vs MdBV) injection on L5 Spodoptera frugiperda larvae hemocytes transcriptome.
2011-04-05 | GSE16775 | GEO
Project description:Comparison of the effects of 2 polydnaviruses (HdIV vs MdBV) injection on L5 Spodoptera frugiperda larvae fat body transcriptome.
2011-04-05 | GSE16776 | GEO
Project description:Peptide fingerprinting to verify the efficacy of phosphatase inhibitor beads (PIBs) for capturing Spodoptera frugiperda (Sf9) phosphatases of the PP2A family.
2025-03-13 | PXD045891 | Pride
Project description:In this study, NanoString technology gene expression quantification platform was used to study the expression of toxin genes causing infections from Bacteria (Photorhabdus and Xenorhabdus), Nematode (H.indica, S.riobrave , S.carpocapsae) specific genes for detection and Immune related genes from the infected insects (Spodoptera frugiperda and Galleria mellonella). The study revealed the expression of different immune related genes from the infected insects (Spodoptera frugiperda and Galleria mellonella) and helped in understanding the trend of expression of gene in the samples from the healthy condition to the death stage. Variations in gene expression were seen as per the expectation.
2024-08-31 | GSE197737 | GEO