ABSTRACT: Raw RNA sequencing data of the fungus in the transcriptomic study on the interaction between Salvia miltiorrhiza and Trichoderma atroviride D16 under cadmium-contaminated conditions
Project description:In the present study we have assessed, by transcriptional profiling, the systemic defense response of Zea mays plants to the ear rotting pathogen Fusarium verticilioides induced by the beneficial fungus Trichoderma atroviride
Project description:A self-designed Trichoderma high density oligonuclotide (HDO) microarray (Roche-NimbleGen, Inc., Madison, WI, USA) was constructed in a similar way than a previous Trichoderma HDO microarray (Samolski et al., 2009). The microarray was composed of 392,779 60-mer probes designed against 13,443 EST-derived transcripts (Trichochip-1) and the genomes of T. atroviride (11,100 genes) and T. virens (11,643 genes). The Trichochip-1 ESTs were obtained from 28 cDNA libraries from eight different species (representing the biodiversity of this genus: T. harzianum, T. atroviride, T. asperellum, T. viride, T. longibrachiatum, T. virens, T. stromaticum and T. aggresivum) under a wide range of growth conditions, including biocontrol-related conditions and different nutritional situations (VizcaÃno et al., 2006). The Trichochip1 EST database was generated in the TrichoEST project funded by the EU (QLK3-CT-2002-02032) T. atroviride P1 mycelium grown (approximately for 24h) at 25ºC on a cellophane sheet on PDA (Difco) plates before contact (at a distance of 5 mm) a R. solani colony grown under identical conditions in the same plate was compared with T. atroviride P1 mycelium after contact (5 mm) the above R. solani colony (mycoparasitic interaction). RNAs from both conditions were extracted and the corresponding cDNAs were use to hybridize by triplicate the Trichoderma HDO microarray.
Project description:This study investigates the multifaceted defense mechanisms of plants in response to beneficial fungi Trichoderma atroviride. The researchers focus on a small RNA, Ta_sRNA1, produced by T. atroviride during its interaction with Arabidopsis thaliana.
Project description:Trichoderma species promote growth and strengthen immunity of Arabidopsis and crop species through multiple mechanisms. However, how fungal proteins mediate growth-defense tradeoffs is unknown. We analyzed the growth, root architecture, defense and global gene expression profiles in Arabidopsis seedlings co-cultivated with T. atroviride WT, and Δnox1, Δnox2, and ΔnoxR mutants, defective on the catalytic and regulatory subunits of NADPH oxidase, respectively. The gene expression profile in the fungus was also characterized in standard growth conditions and in the presence of plants. The results revealed the critical role of Trichoderma NoxR in mediating growth-defense tradeoffs in Arabidopsis. The effects of T. atroviride WT in improving root branching and biomass production decreased in all three related NADPH defective mutants, particularly in ΔnoxR. In contrast, induction of jasmonic acid-related defense responses in roots and shoots were exacerbated in ΔnoxR compared to the WT strain. Transcriptome analyses showed a tight plant-fungus communication based on reactive oxygen species and availability of carbon resources. The ΔnoxR is unable to perceive changes in nutrient sources and activate signaling cascades, which suppresses the metabolic change from saprophyte to commensal. Thus we conclude that Trichoderma NoxR orchestrates fungal-induced development and defense tradeoffs in Arabidopsis and plays an important role in cross-kingdom plant-fungus communication.
Project description:A Trichoderma microarrays composed of 385,000 probes, designed against the genomes of Trichoderma reesei (= Hypocrea jecorina), ID: 431241 (9,129 genes) + Trichoderma virens (= Hypocrea virens), ID: 413071 (11,643 genes) + Trichoderma atroviride (= Hypocrea atroviridis) ID: 197014A (11,643 genes), was constructed (Roche-NimbleGen, Inc., Madison, WI, USA). Probes contained entere transcript sequence. This microarray was used to analyze the transcriptomic changes of T. atroviride IMI 352941 (T11) in three conditions: T11 growing alone, T11 growing at ca 5 mm of V. dahliae V-138I and T11 overgrowing V-138I.
Project description:Trichoderma atroviride is a fungus capable of establishing symbiotic relationships with plants, however, its main lifestyle is a saprophyte. Due to these characteristics, it must face a great quantity of microorganisms, and be able to compete for nutrients. T. atroviride is considered a necrotrophic mycoparasite and has developed the ability to kill other organisms and obtain nutrients from them. The object of this work is to explore the role of small RNAs in mycoparasitism. To this end, we obtained small RNA-Seq libraries from the interactions of T. atroviride against Alternaria alternata. The libraries were obtained from three stages during mycoparasitsm: before contact (BC), contact (C), and after contact (AC).
Project description:Trichoderma atroviride is a fungus capable of establishing symbiotic relationships with plants, however, its main lifestyle is a saprophyte. Due to these characteristics, it must face a great quantity of microorganisms, and be able to compete for nutrients. T. atroviride is considered a necrotrophic mycoparasite and has developed the ability to kill other organisms and obtain nutrients from them. The object of this work is to explore the role of small RNAs in mycoparasitism. To this end, we obtained RNA-Seq libraries from the interactions of T. atroviride against Alternaria alternata and Rhizoctonia solani. The transcriptomes were obtained from three stages during mycoparasitsm: before contact (BC), contact (C), and after contact (AC).
Project description:Phosphite (Phi) is widely used in agriculture due to its biostimulant effects on plants and its ability to control various phytopathogens. However, its impact on beneficial soil microorganisms remains poorly understood. In this study, we evaluated the effect of Phi on the growth and transcriptional response of the beneficial fungus Trichoderma atroviride. Our results show that low concentrations of Phi, in the presence of phosphate (Pi), promote the growth of T. atroviride, whereas higher concentrations inhibit its development. Transcriptomic analysis via RNA-Seq revealed the activation of genes associated with growth, amino acid biosynthesis, and siderophore transport. Furthermore, Phi enhanced the antagonistic capacity of T. atroviride against Rhizoctonia solani. These findings reveal a novel role of Phi in stimulating beneficial fungi and suggest its combined use with T. atroviride as a sustainable strategy for phytopathogen biocontrol in agricultural systems.
