Project description:Lignocellulosic biomass is still considered a feasible source of bioethanol production. Saccharomyces cerevisiae can adapt to detoxify lignocellulose-derived inhibitors, including furfural. Tolerance of strain performance has been measured by the extent of the lag phase for cell proliferation following the furfural inhibitor challenge. The purpose of this work was to obtain a tolerant yeast strain against furfural through overexpression of YPR015C using the in vivo homologous recombination method. The physiological observation of the overexpressing yeast strain showed that it was more resistant to furfural than its parental strain. Fluorescence microscopy revealed improved enzyme reductase activity and accumulation of oxygen reactive species due to the harmful effects of furfural inhibitor in contrast to its parental strain. Comparative transcriptomic analysis revealed 79 genes potentially involved in amino acid biosynthesis, oxidative stress, cell wall response, heat shock protein, and mitochondrial-associated protein for the YPR015C overexpressing strain associated with stress responses to furfural at the late stage of lag phase growth. Both up- and down-regulated genes involved in diversified functional categories were accountable for tolerance in yeast to survive and adapt to the furfural stress in a time course study during the lag phase growth. This study enlarges our perceptions comprehensively about the physiological and molecular mechanisms implicated in the YPR015C overexpressing strain's tolerance under furfural stress. Construction illustration of the recombinant plasmid. a) pUG6-TEF1p-YPR015C, b) integration diagram of the recombinant plasmid pUG6-TEF1p-YPR into the chromosomal DNA of Saccharomyces cerevisiae.

| S-EPMC10266292 | biostudies-literature

Saccharomyces cerevisiae strain:BY4741/BY4742

Project description:High throughput yeast replicative lifespan screen uncovers histone deacetylase complex HDA as novel regulator of aging

| PRJNA601478 | ENA

Saccharomyces cerevisiae

Project description:Genomic saturation mutagenesis in Saccharomyces cerevisiae for isolation of mutants in non-selectable and polygenic traits

| PRJNA255451 | ENA

Saccharomyces cerevisiae BY4741

Project description:Improving the Z3EV promoter system to create the strongest yeast promoter

| PRJDB18827 | ENA

Saccharomyces cerevisiae promoter library

Project description:Combinatorial promoter expression level estimation via cell sorting The purpose of this experiment was to determine the expression level of a library of synthetic promoters. The promoters were cloned in front of a GFP reporter and the resulting library transformed into yeast, sorted by FACS into six fluorescence bins, and the contents of the bins sequenced to determine the distribution of each promoter among each fluorescence bin. This was then used to calculate an expression level for each promoter with enough data.

2013-09-01 | GSE48860 | GEO

Saccharomyces cerevisiae

Project description:Two Distinct Promoter Nucleosome Architectures at Protein-Coding Genes in Yeast

| PRJNA296702 | ENA

Saccharomyces cerevisiae strain:BY4742

Project description:Saccharomyces cerevisiae strain:BY4742 Raw sequence reads

| PRJNA1001843 | ENA