Project description:These data belong to a metabolic engineering project that introduces the reductive glycine pathway for formate assimilation in Cupriavidus necator. As part of this project we performed short-term evolution of the bacterium Cupriavidus necator H16 to grow on glycine as sole carbon and energy source. Some mutations in a putiative glycine transporting systems facilitated growth, and we performed transcriptomics on the evolved strain growing on glycine. Analysis of these transcriptomic data lead us to the discovery of a glycine oxidase (DadA6), which we experimentally demonstrated to play a key role in the glycine assimilation pathay in C. necator.
Project description:The genome of Cupriavidus necator H16 encodes multiple homologous enzymes predicted to be involved in itaconic acid metabolism. Here, we performed RNA-seq analysis to identify genes and gene clusters that are differentially expressed in response to itaconic acid, as well as the metabolically related compounds methylsuccinic acid and mesaconic acid.
