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Spatiomolecular mapping reveals anatomical organization of heterogeneous cell types in the human nucleus accumbens [snRNA-seq]

ABSTRACT: Spatiomolecular mapping reveals anatomical organization of heterogeneous cell types in the human nucleus accumbens [snRNA-seq]

PROVIDER: PRJNA1322344 | ENA |

REPOSITORIES: ENA

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Spatiomolecular mapping reveals anatomical organization of heterogeneous cell types in the human nucleus accumbens

Project description:Spatiomolecular mapping reveals anatomical organization of heterogeneous cell types in the human nucleus accumbens

| PRJNA1440358 | ENA

Spatiomolecular mapping reveals anatomical organization of heterogeneous cell types in the human nucleus accumbens [Spatial Transcriptomics]

Project description:Spatiomolecular mapping reveals anatomical organization of heterogeneous cell types in the human nucleus accumbens [Spatial Transcriptomics]

| PRJNA1322348 | ENA

Spatiomolecular mapping reveals anatomical organization of heterogeneous cell types in the human nucleus accumbens [snRNA-seq]

Project description:The nucleus accumbens (NAc) is a key component of the mesolimbic dopamine system that critically regulates many behaviors related to reward and motivation. The NAc is implicated in several neuropsychiatric disorders, including major depressive disorder, schizophrenia, and substance use disorder. Rodent studies have identified spatial organization of heterogeneous medium spiny neuron (MSN) subtypes across the NAc core and shell, but the extent to which this cellular diversity and spatial organization is conserved in the human brain remains unclear. Here, we generated a spatiomolecular atlas of NAc cell types and spatial domains by integrating spatial transcriptomics and single-nucleus RNA sequencing data from postmortem NAc tissue from 10 neurotypical adult donors. We identified 20 transcriptionally unique cell populations and 8 spatial domains, including specialized D1 islands composed of distinct dopamine receptor 1 (DRD1) MSN subtypes, which were enriched for OPRM1. In contrast to a discrete core vs. shell division, we observed continuous spatial gradients of gene expression across MSN domains, suggesting a more complex organization of DRD1 and DRD2 MSNs. Cross-species comparisons demonstrated conservation of MSN subtypes and spatial features between human, rodent, and nonhuman primate NAc. Genetic enrichment analysis with stratified linkage disequilibrium score regression revealed specific spatial domains associated with risk for psychiatric and addiction-related traits. To investigate this further, we spatially mapped ligand-receptor interactions involving neuropsychiatric risk genes. Finally, we leveraged existing rodent NAc data to identify drug-responsive transcriptional programs and predict their spatial distribution in the human NAc. Collectively, we provide a spatiomolecular framework for understanding the human NAc and its relevance to neuropsychiatric disease.

2025-09-10 | GSE307587 | GEO

Spatiomolecular mapping reveals anatomical organization of heterogeneous cell types in the human nucleus accumbens [Spatial Transcriptomics]

2025-09-10 | GSE307586 | GEO

Spatiomolecular mapping reveals anatomical organization of heterogeneous cell types in the human nucleus accumbens

Project description:The primary goal of the Xenium experiments was to understand the cell type composition of D1-islands identified with Visium spatial transcriptomics, and to evaluate gradients of gene expression across the NAc.

2026-03-30 | GSE325489 | GEO

Molecular and anatomical organization of the dorsal raphe nucleus

Project description:The dorsal raphe nucleus (DRN) is an important source of neuromodulators and has been implicated in a wide variety of behavioral and neurological disorders. The DRN is subdivided into distinct anatomical subregions comprised of multiple cell types, and its complex cellular organization has impeded efforts to investigate the distinct circuit and behavioral functions of its subdomains. Here we used single-cell RNA sequencing, in situ hybridization, anatomical tracing, and spatial correlation analysis to map the transcriptional and spatial profiles of cells from the mouse DRN. Our analysis of 39,411 single-cell transcriptomes revealed at least 18 distinct neuron subtypes and 5 serotonergic neuron subtypes with distinct molecular and anatomical properties, including a serotonergic neuron subtype that preferentially innervates the basal ganglia. Our study lays out the molecular organization of distinct serotonergic and non-serotonergic subsystems, and will facilitate the design of strategies for further dissection the DRN and its diverse functions.

2019-08-06 | GSE134163 | GEO

Molecular, anatomical, and functional organization of lung interoceptors

Project description:Molecular, anatomical, and functional organization of lung interoceptors

| PRJNA772785 | ENA

Molecular and anatomical organization of the dorsal raphe nucleus

Project description:Molecular and anatomical organization of the dorsal raphe nucleus

| PRJNA554121 | ENA

Identification of U1 snRNA associated proteins

Project description:In this project, we isolate U1 snRNA associated proteins in Arabidopsis thaliana. We used an antisense oligonucleotide specific for the U1 snRNA and analyzed associated proteins by mass spectrometry. As a control, the same experiments were performed with U2 snRNA- and lacZ-specifc antisense oligonucleotides.

2023-12-04 | PXD045484 | Pride

Mapping genotype-expression associations in Heterogeneous Stock rat brains to advance behavioral genetics research [Nucleus accumbens core]

Project description:Mapping genotype-expression associations in Heterogeneous Stock rat brains to advance behavioral genetics research [Nucleus accumbens core]

| PRJNA723870 | ENA

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