Project description:In the present experiments, we used in vitro cultured bovine lymphocytes infected with T. annulata to purify the cycloartilaginous stage shizonts of T. annulata and the cycloartilaginous stage merozoites of T. annulata after using T. annulata-infected erythrocytes from live cows (with 30% staining rate), and then used the Proteomics and phosphorylated proteomics data of the two stages of T. annulata were measured on a Thermo Orbitrap Astral mass spectrometer for mass spectrometry uptake and resolution.
Project description:Extracellular vesicles (EVs) are key mediators of intercellular communication, and often play critical roles in host-parasite interactions by facilitating parasite’s physiology and pathogenesis. Theileria annulata, an apicomplexan parasite, induces profound changes in host cells, leading to uncontrolled proliferation, apoptosis resistance, and increased invasiveness. In this study, we performed the comprehensive proteomic and small RNA analysis of EVs isolated from a T. annulata Kashi isolate-infected bovine lymphocyte cell line (TaXJS), B cell line (TaBC), dendritic cell line (TaDC), and from the sera of cattle before and after infection. Our label-free LC-MS/MS proteomics identified 2580 proteins, while small RNA sequencing revealed 6635 miRNAs associated with parasite development, host invasion, and immune evasion. Functional enrichment analyses recognized vesicular components involved in key pathways of the parasite-host such as ECM-receptor interaction, oxidative phosphorylation, and proton transport. These findings highlight the potential of Theileria-derived EVs in modulating host responses and their potential as therapeutic and vaccine targets.
