Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Homo sapiens

ABSTRACT: Genomic Collaboration of Estrogen Receptor-α and ERK2 in Regulating Gene and Proliferation Programs

OTHER RELATED OMICS DATASETS IN: E-GEOD-24592

PROVIDER: PRJNA132379 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

E-GEOD-24592

Project description:Genomic Collaboration of Estrogen Receptor- and ERK2 in Regulating Gene and Proliferation Programs

2025-01-24 | E-GEOD-24592 | ExpressionAtlas

Genomic Collaboration of Estrogen Receptor-α and ERK2 in Regulating Gene and Proliferation Programs

Project description:The nuclear hormone receptor, estrogen receptor-alpha (ERα), and MAP kinases both play key roles in hormone-dependent cancers, yet their interplay and the integration of their signaling inputs remain poorly understood. In these studies, we document that estrogen-occupied ERα activates and interacts with ERK2, a downstream effector in the MAPK pathway, resulting in ERK2 and ERα colocalization at chromatin binding sites across the genome of breast cancer cells. KEYWORDS: siRNA knock-down, ligand treatment

2011-01-10 | GSE24592 | GEO

Genomic Collaboration of Estrogen Receptor-Î± and ERK2 in Regulating Gene and Proliferation Programs

Project description:The nuclear hormone receptor, estrogen receptor-alpha (ERÎ±), and MAP kinases both play key roles in hormone-dependent cancers, yet their interplay and the integration of their signaling inputs remain poorly understood. In these studies, we document that estrogen-occupied ERÎ± activates and interacts with ERK2, a downstream effector in the MAPK pathway, resulting in ERK2 and ERÎ± colocalization at chromatin binding sites across the genome of breast cancer cells. KEYWORDS: siRNA knock-down, ligand treatment MCF-7 human breast adenocarcinoma cells were tranfected with control, ERK1 and ERK2 siRNA for 60 hours and treated with 0.1% EtOH (Vehicle) or 10 nM E2 for 4 hours or 24 hours, and cDNA microarray analyses were carried out using Affymetrix [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array.

2011-01-10 | E-GEOD-24592 | biostudies-arrayexpress

Homo sapiens

Project description:Cross-talk between Estrogen Receptor-related Receptor alpha (ERRalpha) and Estrogen Receptor (ERalpha) pathways.

| PRJNA106665 | ENA

Homo sapiens

Project description:An Estrogen Receptor α-bound Human Chromatin Interactome

| PRJNA119301 | ENA

Homo sapiens

Project description:Active estrogen receptor-alpha signaling in ovarian cancer

| PRJNA322076 | ENA

Homo sapiens

Project description:The Estrogen receptor alpha cistrome defined by DamIP

| PRJNA148121 | ENA

Homo sapiens

Project description:Combinatorial regulation of estrogen receptor alpha-responsive promoters

| PRJNA94257 | ENA

Cooperative interaction between retinoic acid receptor-alpha and estrogen receptor in breast cancer.

Project description:Retinoic acid receptor-alpha (RAR alpha) is a known estrogen target gene in breast cancer cells. The consequence of RAR alpha induction by estrogen was previously unknown. We now show that RAR alpha is required for efficient estrogen receptor-alpha (ER)-mediated transcription and cell proliferation. RAR alpha can interact with ER-binding sites, but this occurs in an ER-dependent manner, providing a novel role for RAR alpha that is independent of its classic role. We show, on a genome-wide scale, that RAR alpha and ER can co-occupy regulatory regions together within the chromatin. This transcriptionally active co-occupancy and dependency occurs when exposed to the predominant breast cancer hormone, estrogen--an interaction that is promoted by the estrogen-ER induction of RAR alpha. These findings implicate RAR alpha as an essential component of the ER complex, potentially by maintaining ER-cofactor interactions, and suggest that different nuclear receptors can cooperate for effective transcriptional activity in breast cancer cells. RAR alpha silenced breast cancer MCF-7 cell lines or control siRNA in the presence of estrogen or a vehicle. MCF-7 cells were hormone-depleted for 3 d and treated with 100 nM estrogen for 12 h. There were three biological replicates for each of the four different groups.

2010-06-16 | E-GEOD-22213 | biostudies-arrayexpress

Homo sapiens

Project description:Coativator Function Defines the Active Estrogen Receptor Alpha Cistrome

| PRJNA121689 | ENA