ABSTRACT: Co-culture of unialgal cyanobacterium with co-existing heterotrophic bacterium from Dresden University of Technology, Germany - Nostoc_73.1/Streptobacillus metagenome
Project description:Co-culture of unialgal cyanobacterium with co-existing heterotrophic bacterium from Dresden University of Technology, Germany - Nostoc_65.1/Streptobacillus metagenome
Project description:Co-culture of unialgal cyanobacterium with co-existing heterotrophic bacterium from Dresden University of Technology, Germany - Nostoc_1.3/Streptobacillus metagenome
Project description:Co-culture of unialgal cyanobacterium with co-existing heterotrophic bacterium from Dresden University of Technology, Germany - Nostoc_62.1/Streptobacillus metagenome
Project description:Co-culture of unialgal cyanobacterium with co-existing heterotrophic bacterium from Dresden University of Technology, Germany - Nostoc_40.5/Streptobacillus metagenome
Project description:Co-culture of unialgal cyanobacterium with co-existing heterotrophic bacterium from Dresden University of Technology, Germany - Calothrix_61.4/Streptobacillus metagenome
Project description:Contaminated culture of unialgal cyanobacteria from Dresden University of Technology, Germany - Nostoc_microscopicum_S25 metagenome
Project description:For the filamentous cyanobacterium Anabaena variabilis to grow without combined nitrogen, certain cells differentiate into heterocysts that fix N2, while vegetative cells perform photosynthesis. Much remains unknown on how heterocysts differ from vegetative cells in terms of carbon and energy metabolisms. Microarrays were used to investigate gene transcription patterns in vegetative cells, heterocysts, and filaments of N2-fixing phototrophic, mixotrophic, and heterotrophic cultures.
Project description:In their natural environments, microbes rarely exist in isolation; instead, they thrive in consortia where diverse interactions take place. In this study, a defined synthetic co-culture of the cyanobacterium S. elongatus cscB, which feeds the heterotrophic P. putida cscRABY with sucrose, was investigated to identify potential interactions. In initial experiments, a remarkable growth-promoting effect brought about by the presence of the heterotrophic partner on the cyanobacterium was observed, leading to a growth rate increase of up to 80% and increased photosynthetic capacity. Vice versa, the cyanobacterium had a neutral effect on P. putida cscRABY, highlighting the resilience against stress of Pseudomonads and their potential as co-culture partners. A suitable reference process reinforcing the growth-promoting effect was established in a parallel operating photobioreactor system, which sets the basis for the analysis of the co-culture at transcriptional, proteomic, and metabolomic level. This multi-OMICs approach revealed several moderate changes, including alterations in the metabolism, transportation, and stress response in both microbes, indicating multi-level interactions. These findings provide valuable insights into the complex dynamics within the co-culture system, suggesting the exchange of further molecules beyond the unidirectional feeding with sucrose.
