Project description:We applied an iTRAQ-based quantitative proteomic approach to understand the differential proteome expression of marine diatom Skeletonema dohrnii grown in different temperature and silicate concentration.
Project description:The comparision between gradual ocean acidification (GC) and one way ocean acidification (HC) of physiological and molecular responses on diatom Skeletonema costatum
Project description:Rising atmospheric CO2 concentrations are leading to ocean acidification, altering the inorganic carbon buffer system with consequences for marine organisms. Here we applied RNA-seq and iTRAQ quantification to investigate the potential impacts of ocean acidification on the temperate coastal marine diatom Skeletonema marinoi.
Project description:We applied an iTRAQ-based quantitative proteomic approach to compare the protein expressionprofiles of Skeletonema dohrnii grown in lower silicate and temperature conditions.
Project description:Purpose:Using Illumina high-throughout sequencing technology, the transcriptome and expression profiles of S. marinoi were sequenced and analyzed. Comprehensive and systematic study of transcriptomic characteristics, analysis of characteristics of Digital Gene Expression Profiling during different growth phase and deep exploration of molecular mechanism for response to the temperature and low silicate stress environment will provide a comprehensive insight into gene expression profiles and provides an important gene resource for future genetic and molecular studies of S. marinoi and other Skeletonema family diatom.
Project description:The experiment was designed to study the transcriptomic response of the centric diatom Skeletonema marinoi to the presence of cues from the grazer Calanus finmarchicus. Samples were collected in triplicate after 65 and 89 hours of exposure of diatom cells to the copepods. Control samples with no copepods were collected at the same time points.
Project description:Studies have demonstrated that marine phytoplankton can adapt to the warmer environment. However, the underlying mechanisms remain largely unknown. Here, we quantified the capacity of a globally distributed marine diatom Skeletonema dohrnii, for rapid evolution under the moderate (24 ℃) and severe (28 ℃) warming scenarios. Whole-genome re-sequencing analysis revealed that the evolutionary adaptation of S. dohrnii to moderate warming was slow (i.e., 700 generations), whereas it was rapid (i.e., 300 generations) under severe warming but suffered a substantial loss of genetic diversity within the population. Genes associated with energy production and lipid metabolism evolved rapidly, particularly under severe warming, suggesting their vital roles in thermal adaptation. Proteomic results also showed the enhanced expression of proteins involved in energy production and lipid metabolism, especially under severe warming. Furthermore, particulate organic carbon and nitrogen production was greatly enhanced in the moderate warming-selected population but increased insignificantly in the severe warming-selected population, indicating more rapid adaptation driven by severe warming. Our results provide molecular insights into the rapid but limited evolution of thermal adaptation in marine diatoms and highlight energy production and lipid metabolism as the most important adaptive strategy. Future warming will affect genetic diversity and population dynamics of diatoms in the ocean.
