Project description:Root traits are significant targets for breeding stress-resilient and high-yielding wheat genotypes under climatic fluctuations. However, root transcriptome analysis is usually obscured due to challenges in root research. We performed transcriptome analysis of thirty bread wheat cultivars using RNA-seq to investigate the diversity and expression of root system architecture (RSA) related transcripts. We examined the expression patterns of these transcripts in both root and leaf tissues and found that various transcripts are root-specific which could be manipulated for desirable root traits.The presented RNA-seq datasets provide valueable source for identification of genes involved in various biological processes under varying climatic conditions.
Project description:The combined effects of high temperature stress from global warming and phosphorus (P) deficiency due to limited P rock reserves pose a critical threat to modern agriculture. However, the understanding of the effect of the combined stress on plants are limited. Here, we performed hydroponic culture to investigate the physiological and transcriptional response of the model legume Lotus japonicus to simultaneous moderate high temperature and low P conditions. While both elevated temperature and low P individually reduced shoot biomass, their combination alleviated growth reduction, with greater shoot biomass observed under combined stress than under low P alone. Lotus japonicus enhanced root-secreted acid phosphatase activity in response to low P alone and increased organic acid exudation rate, such as malate and citrate, under elevated temperature, regardless of P supply. We explored root transcriptome, and detected an up-regulated PEPC1 gene, encoding phosphoenolpyruvate carboxylase which suggested to be related with organic acid root exudation. Additionally, elevated temperature also increased metal ion translocation and absorption, anthocyanin levels, and carbohydrate transport in shoots. Transcriptomic analysis revealed that low P stress altered heat shock protein (HSP) expression, likely due to reduced ATP availability. This study demonstrates that L. japonicus employs distinct and overlapping strategies to adapt to the combined stresses of elevated temperature and P deficiency, including adjustments in exudation patterns, nutrient transport, and stress-responsive gene expression.
2025-12-31 | GSE300599 | GEO
Project description:Transcriptome data of elephant grass under low-temperature stress
| PRJNA1007572 | ENA
Project description:Transcriptome data of Gracilariopsis lemaneiformis under low temperature stress
| PRJNA716486 | ENA
Project description:Transcriptome data of Coffea arabica under low-temperature stress
Project description:Transcriptional profiling of 4 maize varieties comparing genetic root response under control temperature conditions with genetic root response under low temperature conditions
