Project description:This SuperSeries is composed of the following subset Series: GSE24446: Genetic abnormalities in GBM brain tumors GSE24452: Genetic abnormalities in various cell subpopulations of GBM brain tumors GSE24557: Exon-level expression profiles of GBM brain tumors Refer to individual Series
Project description:Glioblastoma (GBM) is the most common primary brain tumor characterized by extensive infiltration into peritumoral brain tissue. GBM tumors exhibit substantial intratumoral heterogeneity making it difficult to identify and target invasive cell subpopulations. In this study, an interface hydrogel model matching in vivo tissue stiffness was utilized to investigate transcriptomic changes in GBM clonal subpopulations as cells migrate across mechanical interfaces. Using single-cell RNA sequencing combined with clonal lineage tracing, unique migratory clonal subpopulations were identified that preferentially migrate at the interface. These cells exhibit a distinct pre-invasive transcriptomic profile characterized by overexpression of galectin-1, a β-galactosidase binding protein. Our findings reveal overexpression of galectin-1 is an innate characteristic of the migratory cells and that expression level is correlated to invasion rate. This study provides insight into the mechanobiological mechanisms underlying GBM invasion and potential therapeutic targets for the invasive population.
Project description:To elucidate the epithelial cell diversity within the nasal inferior turbinates, a comprehensive investigation was conducted comparing control subjects to individuals with house dust mite-induced allergic rhinitis. This study aimed to delineate the differential expression profiles and phenotypic variations of epithelial cells in response to allergic rhinitis. This research elucidated distinct subpopulations and rare cell types of epithelial cells within the nasal turbinates, discerning alterations induced by allergic rhinitis. Furthermore, by interrogating transcriptomic signatures, the investigation provided novel insights into the cellular dynamics and immune responses underlying allergic rhinitis pathogenesis
Project description:Small extracellular vesicles (EVs) have gained attention in the neurology field due to their role in cell-to-cell communication and their potential diagnostic and therapeutic applications. Despite progress in the field, there remains a gap in our understanding of the composition and function of EVs in brain tumours. Previous studies have primarily evaluated EVs obtained from patient fluids or cell cultures, rather than directly from the tumour tissue.
Here we successfully isolated EVs from biopsies of glioblastoma (GBM) or meningioma (MNG) tumors obtained during surgery, marking the first report of in situ EV isolation from brain tumors. The protein content of the tumors and their EVs was characterized using tandem mass spectrometric proteomics, revealing proteins exclusively detected or enriched in EVs relative to tumor. While our study confirmed the presence of proteins previously identified in GBM and MNG EVs from various sources, it also identified novel proteins and pathways associated with EVs from these tumour types. This study underscores the benefit of analyzing in situ EVs direct from brain tissue for insights into tumor biology and highlights the need for further research comparing various types and grades of brain tumors.