Project description:Comparison of gene expression across 85 cell lines after stimulation with a cocktail of inflammatory cytokines Total RNA extracted from Genotyped Lymphoblastoid cell lines under inflammatory and baseline conditions. 3 biological replicates of stimulated and 2 biological replicates of baseline samples were hybridized to the Illumina Human WG-6 v.3 gene expression chip.
Project description:Asthma is a chronic inflammatory airway disease characterized by airway inflammation and remodeling. The role of 15-oxo-5Z,8Z,11Z,13E-eicosatetraenoic acid (15-oxoETE), a 15-HETE metabolite catalyzed by 15-prostaglandin dehydrogenase (15-PGDH), has been relatively unexplored in asthma. In this study, we used RNA-seq to explore the effect of 15-KETE on the transcriptome of airway epithelial cells, aiming to identify its potential downstream targets and mechanisms of action.
