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Mus musculus

ABSTRACT: Polycomb Protein Ezh1 Promotes RNA Polymerase II Elongation

PROVIDER: PRJNA134029 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR073685.fastq.gz	Fastqsanger.gz
	SRR073686.fastq.gz	Fastqsanger.gz
	SRR073687.fastq.gz	Fastqsanger.gz
	SRR073688.fastq.gz	Fastqsanger.gz
	SRR073689.fastq.gz	Fastqsanger.gz

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Similar Datasets

Polycomb Protein Ezh1 Promotes RNA Polymerase II Elongation

Project description:Polycomb group (PcG) proteins initiate the formation of repressed chromatin domains and regulate developmental gene expression. A mammalian PcG protein, Enhancer of Zeste homolog 2 (Ezh2), triggers transcriptional repression by catalyzing the addition of methyl groups onto lysine-27 of histone H3 (H3K27me2/3)1. This action facilitates the binding of other PcG proteins to histone H3 and compaction of chromatin. Interestingly, there exists a paralog of Ezh2, termed Ezh1, whose primary function remains unclear. Here, we provide evidence for genome-wide association of Ezh1 with active epigenetic marks, RNA polymerase II (PolII) and mRNA production. Ezh1 depletion reduced global PolII occupancy within gene bodies and resulted in delayed transcriptional activation during differentiation of skeletal muscle cells. Conversely, ectopic expression of wild-type Ezh1 led to premature gene activation and rescued PolII-elongation defects in Ezh1-depleted cells. Collectively, these findings reveal an unanticipated role of a PcG protein in promoting mRNA transcription. Examination of 3 different histone modifications, 3 modified forms of RNA polymerase II, Ezh1, Ezh2 and mRNA levels in a skeletal muscle cells at various developmental stages.

2011-11-23 | E-GEOD-25549 | biostudies-arrayexpress

Polycomb Protein Ezh1 Promotes RNA Polymerase II Elongation

2011-11-23 | GSE25549 | GEO

EZH1 Orchestrates Rhythmicity of Circadian Gene Expression Acting on Transcription Machinery Integrity

Project description:Circadian rhythmicity of gene expression is a conserved feature of cell physiology. This involves fine tuning between transcriptional and post-transcriptional molecular mechanisms, strongly dependent on the metabolic state of the cell, which guarantees adaptive plasticity of tissue-specific genetic programs. Dynamics of epigenome structure and epigenetic regulators support this plasticity. However, the intermingle between epigenome and RNA Pol II rhythmicity remains to be investigated. Here we identify the Polycomb group (PcG) protein EZH1 as a gateway bridging function regulating periodic alternation between chromatin mediated silencing and active transcription in post-mitotic skeletal muscle cells. We show that PRC2-EZH1 core components are under direct regulation of BMAL1, and show an oscillatory behavior and a regulated periodic assembly of PRC2-EZH1 complex. Instead at alternate Zeitgeber points, EZH1 becomes essential for circadian gene expression, through stabilization of RNA Pol II preinitiation complex controlling nascent transcription process. Collectively, our data show that EZH1 depending on the stoichiometry of its partners guarantees both negative and positive modulation of RNA Pol II activity, resulting in oscillatory transcription.

2024-10-11 | PXD035453 | Pride

Senataxin Promotes RNA Polymerase II Elongation [Hap1_TTseq]

Project description:Senataxin Promotes RNA Polymerase II Elongation [Hap1_TTseq]

| PRJNA1262998 | ENA

Senataxin Promotes RNA Polymerase II Elongation [TTseq]

Project description:Senataxin Promotes RNA Polymerase II Elongation [TTseq]

| PRJNA1175731 | ENA

Senataxin Promotes RNA Polymerase II Elongation [PROseq]

Project description:Senataxin Promotes RNA Polymerase II Elongation [PROseq]

| PRJNA1175732 | ENA

Senataxin Promotes RNA Polymerase II Elongation [RChIPseq]

Project description:Senataxin Promotes RNA Polymerase II Elongation [RChIPseq]

| PRJNA1175733 | ENA

Senataxin Promotes RNA Polymerase II Elongation [DRBrelease_TTseq]

Project description:Senataxin Promotes RNA Polymerase II Elongation [DRBrelease_TTseq]

| PRJNA1175735 | ENA

Senataxin Promotes RNA Polymerase II Elongation [Hap1_DRBrelease_TTseq]

Project description:Senataxin Promotes RNA Polymerase II Elongation [Hap1_DRBrelease_TTseq]

| PRJNA1262997 | ENA

CDK7 kinase activity promotes RNA polymerase II promoter escape by facilitating initiation factor release

Project description:Cyclin-dependent kinase 7 (CDK7), part of the general transcription factor TFIIH, promotes gene transcription by phosphorylating the C-terminal domain of RNA polymerase II (RNA Pol II). Here, we combine rapid CDK7 kinase inhibition with multi-omics analysis to unravel the direct functions of CDK7 in human cells. CDK7 inhibition causes RNA Pol II retention at promoters, leading to decreased RNA Pol II initiation and immediate global downregulation of transcript synthesis. Elongation, termination, and recruitment of co-transcriptional factors are not directly affected. Although RNA Pol II, initiation factors, and Mediator accumulate at promoters, RNA Pol II complexes can also proceed into gene bodies without promoter-proximal pausing while retaining initiation factors and Mediator. Further downstream, RNA Pol II phosphorylation increases and initiation factors and Mediator are released, allowing recruitment of elongation factors and an increase in RNA Pol II elongation velocity. Collectively, CDK7 kinase activity promotes the release of initiation factors and Mediator from RNA Pol II, facilitating RNA Pol II escape from the promoter.

2024-05-31 | PXD037842 | Pride

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