Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Bos taurus

ABSTRACT: Comparison of the bovine embryonic transcriptomes using RNA-Seq

PROVIDER: PRJNA134751 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR072810.fastq.gz	Fastqsanger.gz
	SRR072811_1.fastq.gz	Fastqsanger.gz
	SRR072811_2.fastq.gz	Fastqsanger.gz
	SRR072812.fastq.gz	Fastqsanger.gz
	SRR072813_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Bos taurus

Project description:Profiling microRNA expression in bovine alveolar macrophages using RNA-seq

| PRJNA175910 | ENA

Global mapping of miRNA-target interactions in cattle (Bos taurus)

Project description:With regulatory roles in development, cell proliferation and disease, micro-RNA (miRNA) biology is of great importance and a potential key to novel RNA-based therapeutic regimens. Biochemically based sequencing approaches have provided robust means of uncovering miRNA binding landscapes on transcriptomes of various species. However, a current limitation to the therapeutic potential of miRNA biology in cattle is the lack of validated miRNAs targets. Here, we use cross-linking immunoprecipitation (CLIP) of the Argonaute (AGO) proteins and unambiguous miRNA-target identification through RNA chimeras to define a regulatory map of miRNA interactions in the cow (Bos taurus). The resulting interactome is the deepest reported to date for any species, demonstrating that comprehensive maps can be empirically obtained. We observe that bovine miRNA targeting principles are consistent with those observed in other mammals. Motif and structural analyses define expanded pairing rules with most interactions combining seed-based pairing with distinct, miRNA-specific patterns of auxiliary pairing. Further, miRNA-target chimeras had predictive value in evaluating true regulatory sites of the miR-17 family. Finally, we define miRNA-specific targeting for >5000 mRNAs and determine gene ontologies (GO) for these targets. This confirmed repression of genes important for embryonic development and cell cycle progress by the let-7 family, and repression of those involved in cell cycle arrest by the miR-17 family, but it also suggested a number of unappreciated miRNA functions. Our results provide a significant resource for transcriptomic understanding of bovine miRNA regulation, and demonstrate the power of experimental methods for establishing comprehensive interaction maps.

2017-07-11 | GSE90089 | GEO

Bos taurus

Project description:A comparison of gene expression profiles between glucocorticoid responder and non-responder bovine trabecular meshwork cells using RNA sequencing

| PRJNA315985 | ENA

Bos taurus

Project description:RNA-seq analysis of single bovine blastocysts

| PRJNA188351 | ENA

Bos taurus

Project description:Comparison of the DNA methylation of bovine blastocysts and bovine sperm

| PRJNA248020 | ENA

Bos taurus

Project description:Transcriptomic analysis of the stress response to weaning at housing in bovine leukocytes using RNA-seq technology

| PRJNA160133 | ENA

Bos taurus

Project description:Analysis of the bovine monocyte-derived macrophage response to Mycobacterium avium subspecies paratuberculosis infection using RNA-seq

| PRJNA263027 | ENA

Bos taurus

Project description:Transcriptome analysis of bovine oocytes and small RNA-seq analysis of bovine follicular fluid

| PRJDB6822 | ENA

Bos taurus

Project description:Analysis of microRNAs in bovine early embryonic development

| PRJNA152215 | ENA

Bos taurus

Project description:Comparison of bovine gd and ab T cell clones

| PRJNA92351 | ENA