Project description:ChIP-seq was performed in C. elegans to characterize the genomic binding profiles of LIN-35 (DREAM complex), LIN-15B, and LIN-15A, and to assess their interdependencies. Experiments were conducted in wild-type animals and in lin-15A(fp31), lin-15B(n744), and lin-35(n745) null mutants. All three factors showed highly overlapping binding patterns, predominantly at promoter regions, and shared target genes enriched for metabolic and developmental pathways. Comparative analyses revealed reciprocal effects on binding between LIN-35 and LIN-15B, while loss of LIN-15A primarily resulted in increased LIN-35 and LIN-15B occupancy at a subset of loci, suggesting that LIN-15A modulates DREAM complex binding.
Project description:Transcriptional profiling of C. elegans first larval stage whole animals comparing lin-15B(n744) mutants and N2 (wild type) at 26 degrees. One-condition experiment, mutant (lin-15B) vs. WT. Biological replicates 4 mutant, 4 wildtype harvested indepedently. One lin-35 replicate and one WT replicate per array.
Project description:synMuv B proteins, including LIN-15B, are necessary to repress the expression of germline-promoting genes in the soma during embryonic and early larval development in C. elegans. The goal of this research was to determine if changes to histone modifications could underlie the misexpression of germline genes in these mutants. We performed ChIP-seq on four histone modifications in wild type and three synMuv B mutants at two temperatures to assess these changes.
Project description:Transcriptional profiling of C. elegans first larval stage whole animals comparing lin-15B(n744) mutants and N2 (wild type) at 26 degrees.
