Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA136863 | ENA |
REPOSITORIES: ENA
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Project description:We report genome-wide gene expression changes in olfactory bulb cells, in the context of low, wild-type or high levels of local CRH signaling by local interneurons onto adult-born CRHR1+ granule neurons. To test the gene expression changes associated with altered local CRH signaling, we utilize the following animal models: CRHR1-/- mice whose bulbs were infected with a control AAV-flex-EGFP virus = low local CRH signaling, CRHR1-Cre mice whose bulbs were infected with AAV-flex-EGFP = pseudo-wild-type local CRH signaling, and CRHR1-Cre mice whose bulbs were infected with AAV-flex-(CA)CRHR1, which is a constitutively active variant of CRHR1 = high local CRH signaling. We find that, in response to changes in local CRH signaling in the bulb, the largest ontological category of transcription changes in the bulb that occurs reciprocally between low and high levels of CRH signaling are gene regulatory factors. To test the contributions of one of these factors, POU6f1, we perform loss- and gain-of-function analysis. We show that CRHR1 activation results in transcriptional activation of POU6f1 and that POU6f1 in turn influences synaptogenesis and dendritic patterning of adult-born neurons and olfactory circuit behavior.
2017-10-26 | GSE98799 | GEO
Project description:Purpose: The goal of this study is to investigate the effects of fine particulate matter (PM2.5) exposure on mouse olfactory bulb using next generation sequencing (NGS). Methods: After 28 days of 3 mg/kg/3 day and 10 mg/kg/3 day PM2.5 exposure, olfactory bulb mRNA profiles of 8-week-old wild-type (WT) male C57BL/6 mice were generated by deep sequencing, in 3-4 repeats, using Illumina NovaSeq 6000. For each sample, clean reads were obtained that mapped to mm10 using HISAT2 (hierarchical indexing for spliced alignment of transcripts) v2.0.477. Results: Our study revealed that PM2.5 treatment caused significant effects on the gene expression profilling of mouse olfactory bulb. Overall, the sequencing identified 34,745 transcripts, and two kinds of treatments obtained 60 and 138 differently expressed genes (DEGs) respectively, with a criteria of fold change >2 and q-value <0.5. Most biological events that DEGs involved were inflammation relevant. Conclusions: Our study revealed that PM2.5 treatment caused significant effects on the gene expression profiling of mouse olfactory bulb.
2024-01-22 | GSE186404 | GEO
Project description:The aim of this study was to use global gene expression profiling to define intrinsic molecular differences that distinguish olfactory ensheathing cells from mucosa (OM-OECs) from olfactory ensheathing cells from olfactory bulb (OB-OECs). 10,000 OECs from olfactory mucosa (OM) or olfactory bulb (OB) were isolated from 4 rats.
2009-12-02 | GSE19250 | GEO
Project description:The goal of this study is to profile NFIA DNA-binding properties in the adult mouse brain. We performed chromatin immunoprecipitation of NFIA in the hippocampus and olfactory bulb of wildtype mice, and samples were subjected to sequencing. We find that NFIA preferentially binds DNA in the hippocampus but not in the olfactory bulb as evidenced by the distinct lack of NFIA binding peaks in the olfactory bulb. Mass spectrometry results suggested that NFIA has a significantly higher binding affinity for NFIB in the olfactory bulb, potentially blocking NFIA’s ability to bind DNA. Virally induced siRNAs against NFIB or scramble were injected into the olfactory bulb of adult wildtype mice to knock down NFIB. We performed chromatin immunoprecipitation of NFIA in the olfactory bulb injected with siRNA-NFIB or siRNA-scramble. Subsequent sequencing revealed an increase of NFIA binding in the olfactory bulb upon the depletion of NFIB as compared to the siRNA-scramble and wildtype controls.
2020-02-28 | GSE146064 | GEO
Project description:Emx2 is a homeobox transcription factor that plays a critical role in development. Olfactory sensory neuron axons from Emx2 knockout mice fail to innervate their target tissue, the olfactory bulb. Homeobox transcription factors may also play an important role in olfactory receptor expression. We used microarrays to analyze differences in mRNA abundance in the olfactory epithelium of Emx2 wildtype and knockout embryonic day 18.5 mice. Keywords: Knockout-Wildtype comparision
2008-10-15 | GSE12135 | GEO
Project description:We conducted RNA-Seq and in situ hybridization to identify transcriptional profile changes in the Sp8/Sp9-DCKO olfactory bulb. Simultaneously, we did ChIP-Seq with homemade Sp9 antibody to identify Sp9 binding motifs and which changed genes might be the direct targets of transcription factor Sp9. We found that Sp9 directly binded to key genes which are required for olfactory bulb development. Transcription factors Sp8 and Sp9 belong to the same transcription factor family, so we think Sp8 and Sp9 have very similar binding sites of transcriptional regulation.
2016-09-29 | GSE87416 | GEO
Project description:Ongoing, lifelong neurogenesis maintains the neuronal population of the olfactory epithelium in the face of piecemeal neuronal turnover and restores it following wholesale loss. The molecular phenotypes corresponding to different stages along the progression from multipotent globose basal cell (GBC) progenitor to differentiated olfactory sensory neuron are poorly characterized. We used the transgenic expression of GFP and cell surface markers to FACS-isolate Sox2-GFP(+) GBCs, Neurog1-GFP(+) GBCs and immature neurons, and OMP-GFP(+) mature neurons from normal adult mice. In addition, the latter two populations were also collected 3 weeks after olfactory bulb ablation, a lesion that results in persistently elevated neurogenesis. Global profiling of mRNA from the populations indicates that all stages of neurogenesis share a cohort of >2100 genes that are upregulated compared to sustentacular cells. A further cohort of >1200 genes are specifically upregulated in GBCs as compared to sustentacular cells and differentiated neurons. The increased rate of neurogenesis caused by olfactory bulbectomy had little effect on the transcriptional profile of the Neurog1-GFP(+) population. In contrast, the abbreviated lifespan of OMP-GFP(+) neurons born in the absence of the bulb correlated with substantial differences in gene expression as compared to the mature neurons of the normal epithelium. Detailed examination of the specific genes upregulated in the different progenitor populations revealed that the chromatin modifying complex proteins LSD1 and coREST were expressed sequentially in upstream Sox2-GFP(+) GBCs and Neurog1-GFP(+) GBCs/immature neurons. The expression patterns of these proteins are dynamically regulated after activation of the epithelium by methyl bromide lesion. Total RNA was isolated from dissected, dissociated and FACS-purified olfactory mucosal cells from normal adult mice or mice 3 weeks after unilateral bulbectomy. Cells were purified with FACS using endogenous GFP fluorescence from various transgenic lines and cell surface labeling. Two to seven adult mice were used per replicate and three replicates per condition were performed using Illumina bead arrays. 21 samples from olfactory mucosa were analyzed in this series and 3 samples were from a commercially available reference RNA sample. Universal mouse reference RNA from Stratagene was used as a general control and Normal olfactory mucosa was used as a tissue specific control.
2012-07-01 | E-GEOD-37581 | biostudies-arrayexpress
Project description:Transcription Factors Sp8 and Sp9 Coordinately Regulate Olfactory Bulb Interneuron Development
| PRJNA344717 | ENA
Project description:The phenotypically characterized hTERT immortalized porcine olfactory bulb neuroblast cell line (OBGF400) was subjected to an extensive whole genome-scaled expression profile for establishing their use as an in vitro neuronal disease model system. Microarrays were used to provide a comprehensive knowledge underlying the genomic complexity and overall gene expression capacity of the immortalized OBGF400 cells. The analysis revealed the elaborate signaling mechanisms of this unique subpopulation of porcine neuronally committed progenitors that mirrors the intricate organization of postnatal neurongenic zones. SUBMITTER_CITATION: Transcriptome Profile and Cytogenetic Analysis of Immortalized Neuronally Restricted Progenitor Cells Derived from the Porcine Olfactory Bulb. Animal biotechnology 2009 vol:20 iss:4 page:186-215 Experiment Overall Design: Total cellular RNA extracts from independent OBGF400 (neuroblasts) and PK15 (non-neuronal, epithelial origin) cell cultures (9 each) were pooled into a total of 3 biological replicates per cell line. The concentrations and purity of the pooled RNA preparations were determined using the BioAgilent RNA assay prior to hybridization on Affymetrix GeneChip® Porcine Genome Expression Arrays. To ascertain the genes that were preferentially expressed by the OBGF400 neuroblasts, we used the PK15 cellular array in an effort to exclude somatic cell background.
2009-11-10 | E-GEOD-16855 | biostudies-arrayexpress
Project description:Using the highly sensitive miRNA array, we screened 40 microRNAs abundant in the olfactory bulb and we explored the functions of these miRNAs in the olfactory bulb by Gene Ontology and Kyoto Encyclopedia of Genes annotation. The enrichment results indicated that these miRNAs mainly participated in the axon guidance process. Furthermore, the quantitative real-time polymerase chain reaction, immunohistochemistry, and dual luciferase reporter assay results showed that miR-30c is a specific regulator of semaphorin-3A, which will give new insights in disclosing the mechanism of functional maintenance and sexual-specific differentiation of the olfactory bulb. In this study, three samples from steady-state mice were used to acquire the miRNA expression profiling and the function of the abudant miRNAs in the olfactory bulb were analyzed by bioinformatic methods.Finally, miR-30c was experimentally validated to be a regulator of semaphorin-3A, an important axon guidance cue in the nervous system.
2014-08-27 | E-GEOD-55463 | biostudies-arrayexpress