Danio rerio
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA138173 | ENA |
REPOSITORIES: ENA
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Project description:Acetylcholinesterase (AChE) inhibitors are widely used as pesticides and drugs. Their primary effect is the overstimulation of muscular activity. Whether AChE inhibitors and related neurotoxic compound also provoke specific changes in gene transcription patterns that allow to establish a mechanistic link useful for diagnostic purposes has, however, yet not been investigated. Therefore, as a proof of principle, we examined the transcriptomic response of a known AChE inhibitor, the organophosphate azinphos-methyl (APM), in zebrafish embryos and compared the response with two non-AChE inhibiting control compounds, 1,4-dimethoxybenzene (DMB) and 2,4-dinitrophenol (DNP). A highly specific cluster of APM induced gene transcripts was identified and a subset of strongly regulated genes was analyzed in more detail. The small heat shock protein hspb11 was found to be the most sensitive gene in response to AChE inhibitors. Comparison of expression in wild-type, ache and fixe mutant embryos revealed that hspb11 expression was dependent on the nicotinic acetylcholine receptor (nAChR) activity. Furthermore, modulators of intracellular calcium levels led to an hspb11 transcriptional up-regulation which suggest that elevated intracellular calcium levels and the resulting increase in muscular activity triggering hspb11 up-regulation. During early zebrafish development, hspb11 was specifically expressed in muscle pioneers and morpholino-knockdown resulted in defects in slow muscle myosin organization. These findings demonstrate that a combination of transcriptome and functional analyzes in the zebrafish embryo is able to correlate gene expression changes to the mechanism of action of neurotoxic compounds. Zebrafish embryos were exposed for 24 h and 48 h (26-50 and 2-50 hpf) to 5,83 µM of azinphos-methyl (APM), 509 µM of 1,4-dimethoxybenzene (DMB) and 14,2 µM 2,4-dinitrophenol (DNP), the respective LC10 of each compound, in order to determine mode of action specific responding genes, or not exposed and parallel treated as controls. 50 zebrafish embryos were used per treatment, and 4 biological independent replicates were analysed, respectively.
2011-12-01 | E-GEOD-27680 | biostudies-arrayexpress
Project description:We applied zebrafish whole genome microarrays to identify molecular effects of diazepam, a neuropharmaceutical encountered in wastewater-contaminated environments, and to elucidate its neurotoxic mode of action. Behavioral studies were performed to analyze for correlations between altered gene expression with effects on the organism level. Male zebrafish and zebrafish eleuthero-embryos were exposed for 14 d or up to 3 d after hatching, respectively, to nominal levels of 273 ng/L and 273 μg/L (determined water concentrations in the adult experiment 235 ng/L and 291 μg/L). Among the 51 and 103 altered transcripts at both concentrations, respectively, the expression of genes involved in the circadian rhythm in adult zebrafish and eleuthero-embryos were of particular significance, as revealed both by microarrays and quantitative PCR. The swimming behavior of eleuthero-embryos was significantly altered at 273 μg/L. The study leads to the conclusion that diazepam-induced alterations of genes involved in circadian rhythm are paralleled by effects in neurobehavior at high, but not at low diazepam concentrations that may occur in polluted environments.
2011-07-23 | GSE23157 | GEO
Project description:Concentration dependent gene expression in zebrafish embryos after PCE exposure
| PRJNA269736 | ENA
Project description:We applied zebrafish whole genome microarrays to identify molecular effects of diazepam, a neuropharmaceutical encountered in wastewater-contaminated environments, and to elucidate its neurotoxic mode of action. Behavioral studies were performed to analyze for correlations between altered gene expression with effects on the organism level. Male zebrafish and zebrafish eleuthero-embryos were exposed for 14 d or up to 3 d after hatching, respectively, to nominal levels of 273 ng/L and 273 μg/L (determined water concentrations in the adult experiment 235 ng/L and 291 μg/L). Among the 51 and 103 altered transcripts at both concentrations, respectively, the expression of genes involved in the circadian rhythm in adult zebrafish and eleuthero-embryos were of particular significance, as revealed both by microarrays and quantitative PCR. The swimming behavior of eleuthero-embryos was significantly altered at 273 μg/L. The study leads to the conclusion that diazepam-induced alterations of genes involved in circadian rhythm are paralleled by effects in neurobehavior at high, but not at low diazepam concentrations that may occur in polluted environments. Gene expression in male zebrafish brain was measured after exposure for 14 d to 273 ng/L and 273 ug/L diazepam or to the water control. A total of 11 arrays (Agilent 4 × 44 K Zebrafish microarray) were used, including three for the water control group, four for the 273 ng/L and four for the 273 μg/L diazepam dose group.
2011-07-22 | E-GEOD-23157 | biostudies-arrayexpress
Project description:transcriptome of zebrafish embryos exposed to glyphosate
| PRJNA929522 | ENA
Project description:Gene expression profiling in zebrafish embryos exposed to 3,4-dichloroaniline (3,4-DCA)
| PRJNA97591 | ENA
Project description:Per- and polyfluoroalkyl substances (PFAS) are environmentally persistent chemicals widely detected in aquatic systems and drinking water. Perfluorononanoic acid (PFNA), a long-chain PFAS, has been reported globally in environmental matrices and fish tis-sues. Although PFNA has been linked to developmental, metabolic, and neurological toxicity, its effects on lipid-related pathways and neurotoxicity remain poorly charac-terized. This study evaluated the developmental and neurotoxic effects of PFNA ex-posure in zebrafish embryos and larvae following a 7-day exposure to environmentally relevant PFNA concentrations. PFNA exposure did not significantly affect survival or deformity rates. Apoptosis was significantly increased in larvae exposed to 1 µg/L PFNA compared to controls, whereas reactive oxygen species were unaffected. Each tested concentration (0.1 µg/L and 10 µg/L) showed a different effect on the zebrafish transcriptome. Low-dose PFNA exposure primarily affected lipid transport, choles-terol metabolism, sphingolipid signaling, and neurodegeneration-related pathways, while high-dose PFNA altered synaptic signaling, axon guidance, and thyroid hormone synthesis. Hypoactivity was observed in the movement of larval zebrafish based on a visual motor response test. Taken together, PFNA exposure leads to molecular changes related to neurotoxicity and lipid metabolism in zebrafish, which may con-tribute to adverse neurodevelopmental outcomes.
2026-05-05 | GSE324353 | GEO
Project description:Expression data from zebrafish (Danio rerio) embryos exposed to methyl tert-butyl ether
| PRJNA198857 | ENA