Project description:Identification of EWSR1 and EWS-FLI1 transcript variants associated with the silencing of HNRNPH1 in TC32 Ewing sarcoma and 293T human embryonic kidney (HEK-293T) cells.
Project description:Expression profiles of human embryonic kidney (HEK)-293T cells expressing a GFP (293T-GFP) or a truncated form of Arabidopsis DEMETER (DME) 5-methylcytosine (5mC) DNA glycosylase (293T-DMEΔ) analyzed on an Affymetrix Human Genome U133 Plus 2.0 Array Platform. These array data revealed differentially expressed genes (DEGs) between the 293T-GFP cells (without direct 5mC excision activity) and 293T-DMEΔ cells (with artificially implemented direct 5mC excision activity).
Project description:To investigate the regulation of mRNA by 3'end unridylation, we performed RNA sequencing on HEK 293T cells and its TENT3A/B knockout strains (ΔTENT3A/B). We performed transcriptomic analysis comparing mRNA abundance in HEK 293T cells and ΔTENT3A/B
Project description:Transcription profiling of human umbilical vein endothelial cells overexpressing constitutively active FOXO1 to identify FOXO1 target genes
