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Drosophila melanogaster

ABSTRACT: The tumor suppressor L(3)mbt inhibits neuroepithelial proliferation and acts on insulator elements

PROVIDER: PRJNA140051 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR203277.fastq.gz	Fastqsanger.gz
	SRR203278.fastq.gz	Fastqsanger.gz
	SRR203279.fastq.gz	Fastqsanger.gz
	SRR203280.fastq.gz	Fastqsanger.gz

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Similar Datasets

The tumor suppressor L(3)mbt inhibits neuroepithelial proliferation and acts on insulator elements

Project description:In Drosophila, defects in asymmetric cell division can result in the formation of stem cell derived tumors. Here, we reveal a different mechanism that can result in the formation of very similar terminal brain tumor phenotypes. We demonstrate that brain tumors in l(3)mbt mutants originate from overproliferation of neuroepithelial cells in the optic lobes caused by de-repression of target genes in the Salvador-Warts-Hippo (SWH) pathway. We use ChIP-seq to identify L(3)mbt-binding sites and show that L(3)mbt binds to chromatin insulator elements. Mutating l(3)mbt or inhibiting the insulator protein mod(mdg4) results in upregulation of SWH pathway reporters. As l(3)mbt tumors are rescued by mutations in bantam or yorkie or by overexpression of expanded the deregulation of SWH pathway target genes is an essential step in brain tumor formation. Our data reveal that very different primary defects can result in the formation of brain tumors, which behave quite similarly in their advanced stages.

2011-08-29 | GSE29206 | GEO

The tumor suppressor L(3)mbt inhibits neuroepithelial proliferation and acts on insulator elements

2011-08-29 | E-GEOD-29206 | biostudies-arrayexpress

Drosophila melanogaster

Project description:Distribution of Drosophila insulator proteins Mod(mdg4) and L(3)mbt in Kc cells

| PRJNA153283 | ENA

ATAC-seq analysis of chromatin accessibility and nucleosome positioning in Drosophila melanogaster precellular blastoderm embryos

Project description:Purpose: The goal of this study was to measure how chromatin accessibility and nucleosome positioning changes as embryos undergo the midblastula transition (MBT). Methods: Single or paired embryos were collected at three minute intervals over the three cell cycles leading up to the MBT (nuclear cycles 11, 12, and 13). Embryos were subjected to ATAC-seq library preparation following established protocols. Thirteen total timepoints were collected. At least three biological replicates were collected per timepoint. Samples were subjected to paired end sequencing on an Illumina HiSeq 2500. Wild type (diploid) embryos were compared with haploid (sesame) embryos to determine whether changes in chromatin accessibility are governed by measurement of the nucleo-cytoplasmic ratio, a well known biological timer that controls the onset of the MBT. Results: Chromatin accessibility changes dynamically during the period leading up to the MBT. Initially, genomic enhancer elements are accessible, but during NC12 and NC13, large scale chromatin remodeling results in a gain of accessibility for promoter elements and insulators. A substantial fraction of the dynamic chromatin accessibility is temporally regulated by the mechanism that measures the embryonic nuclear-cytoplasmic ratio, as determined by comparison between haploid and diploid embryos. A substantial fraction of open chromatin regions remain 'accessible' during mitotic metaphase.

2016-11-22 | GSE83851 | GEO

Drosophila melanogaster

Project description:Total mRNA and piRNA sequences of delta-mbt-OSCs

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Drosophila melanogaster

Project description:Tissue-specific regulation of chromatin insulator function

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Drosophila melanogaster

Project description:Distribution of Drosophila insulator proteins during interphase and mitosis

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