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Homo sapiens

ABSTRACT: Epigenetic regulation of cell type-specific expression patterns in the human mammary epithelium [MSDK-Seq]

OTHER RELATED OMICS DATASETS IN: GSE26141

PROVIDER: PRJNA142403 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR088780.fastq.gz	Fastqsanger.gz
	SRR088781.fastq.gz	Fastqsanger.gz
	SRR088782.fastq.gz	Fastqsanger.gz
	SRR088783.fastq.gz	Fastqsanger.gz
	SRR088784.fastq.gz	Fastqsanger.gz

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Homo sapiens

Project description:Epigenetic regulation of cell type-specific expression patterns in the human mammary epithelium [SAGE-Seq]

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Project description:Epigenetic regulation of cell type-specific expression patterns in the human mammary epithelium [ChIP-Seq]

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Epigenetic regulation of cell type-specific expression patterns in the human mammary epithelium

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Epigenetic regulation of cell type-specific expression patterns in the human mammary epithelium [SAGE-Seq]

Project description:Here we describe the application of high-throughput sequencing technology for profiling histone and DNA methylation, and gene expression patterns of normal human mammary progenitor-enriched and luminal lineage-committed cells. We observed significant differences in histone H3 lysine 27 tri-methylation (H3K27me3) enrichment and DNA methylation of genes expressed in a cell type-specific manner, suggesting their regulation by epigenetic mechanisms and a dynamic interplay between the two processes that together define developmental potential. The technologies we developed and the epigenetically regulated genes we identified will accelerate the characterization of primary cell epigenomes and the dissection of human mammary epithelial lineage-commitment and luminal differentiation. Global transcriptome profilings of 15 samples in normal breast tissue using Sage-Seq.

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EZH2 controls mammary differentiation independently of its methyltransferase activity through controlling genomic STAT5 access

Project description:Mammary development is characterized by the proliferation and progressive differentiation of alveolar epithelium during pregnancy, culminating in lactation. These processes are largely controlled by hormones through transcription factors. We now explore the contributions of histone methyltransferases, which establish H3K27me3 marks, in the temporally-regulated differentiation of mammary epithelium. Loss of EZH2, but not EZH1, resulted in precocious mammary differentiation, which was facilitated by STAT5 binding to specific target genes and their activation. Mammary stem cells were not compromised in the absence of EZH2. Genome-wide H3K27me3 patterns remained intact in the absence of EZH2. Mammary-specific loci were devoid of H3K27me3 marks in mammary progenitor and mature cells, suggesting no regulatory role for this repressive mark. Lastly, the combined absence of EZH1 and EZH2 inhibited the formation of alveoli. Taken together, EZH2 controls temporally-restricted differentiation of mammary epithelium through H3K27me3-independent mechanisms. mRNA-seq and ChIP-seq in MMTV-Cre (Control), E1-/- (E1KO), E1+/-;E2f/f;control (E1+/-E2KO) and Ezh2f/f;control (E2KO) mammary gland tissues or MECs (purified mammary epithelial cells). H3K27me3 and STAT5 ChIP-seqs in mammary tissues at p13; H3K4me3 ChIP-seq in MECs (mammary epithelial cells) at p13; RNA-seqs at mature virgin (with/without prolactin injection), p13 and p18 mammary tissues.

2013-12-31 | E-GEOD-52016 | biostudies-arrayexpress

Epigenetic regulation of cell type-specific expression patterns in the human mammary epithelium [MSDK-Seq]

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2011-03-01 | GSE26137 | GEO

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