Project description:Bone is the most common site of breast cancer metastasis, and this type of metastasis is a main cause of morbidity. Because breast cancer is a heterogeneous disease, the interactions between the cancer cells and the skeletal host cells, such as osteoblasts, might be diverse. Thus far, these tumor-osteoblast interactions have not yet been well characterized using a genomic approach. We hypothesized that gene expression signatures induced by tumor-osteoblast interactions might be of clinical relevance. To examine these gene expression signatures, we established an ex vivo co-culture model with benign human cells and a panel of 5 malignant breast epithelial cells in combination with primary human osteoblasts and determined associated gene expression changes using cDNA microarrays. This SuperSeries is composed of the following subset Series: GSE29030: Normal human osteoblasts and HMEC mono and coculture GSE29031: Normal human osteoblasts and Hs578t mono and coculture GSE29032: Normal human osteoblasts and MCF7 mono and coculture GSE29033: Normal human osteoblasts and MDA-MB-231 mono and coculture GSE29034: Normal human osteoblasts and SKBR3 mono and coculture GSE29035: Normal human osteoblasts and T47D mono and coculture Refer to individual Series
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
Project description:Transcriptional profiling of human mesenchymal stem cells comparing normoxic MSCs cells with hypoxic MSCs cells. Hypoxia may inhibit senescence of MSCs during expansion. Goal was to determine the effects of hypoxia on global MSCs gene expression.
