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Mus musculus

ABSTRACT: Conditional knockout of Sox2 in embryonic and trophoblast stem cells.

OTHER RELATED OMICS DATASETS IN: E-GEOD-28451

PROVIDER: PRJNA143237 | ENA |

REPOSITORIES: ENA

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Dynamics of Sox2 occupancy during the differentiation of embryonic stem cells into trophoblast stem cells.

Project description:To understand the mechanism underlying the versatility in transcriptional regulation by Sox2, we compared genome-wide binding sites of Sox2 in embryonic stem (ES) cells and trophoblast stem (TS) cells by chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq). A tetracycline-inducible Oct3/4 knockout ES cell line ZHBTc4 was treated with Tet for 4 days in the presence of FGF4 and mouse embryonic fibroblasts (MEFs).

2013-10-17 | E-GEOD-28453 | biostudies-arrayexpress

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Project description:Dynamics of Sox2 and Esrrb occupancy during the differentiation of embryonic stem cells into trophoblast stem cells.

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Conditional knockout of Sox2 in embryonic and trophoblast stem cells.

2013-10-17 | GSE28451 | GEO

Conditional knockout of Sox2 in embryonic and trophoblast stem cells.

Project description:To compare the transcriptional networks governed by Sox2 in embryonic stem (ES) cells and trophoblast stem (TS) cells, we performed whole-genome expression analysis after tetracycline (Tet)-induced knockout of Sox2 in each cell type. A Tet-inducible Sox2 knockout ES cell line 2TS22C or its derivative TSC line 22CEROH2-TSC1 were treated with Tet for 4 days. A total of 12 samples from ES or TS cells cultured with or without Tet were analyzed in three biological replicates.

2013-10-17 | E-GEOD-28451 | biostudies-arrayexpress

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Project description:Overexpression of trophoblast stem cell-enriched microRNAs promote trophoblast fate in embryonic stem cells.

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Differential requirements for Smarca5 expression during hematopoietic stem cell commitment.

Project description:The formation of hematopoietic cells relies on the chromatin remodeling activities of ISWI ATPase SMARCA5 (SNF2H) and its complexes. The Smarca5 null and conditional alleles have been used to study its functions in embryonic and organ development in mice. These mouse model phenotypes vary from embryonic lethality of constitutive knockout to less severe phenotypes observed in tissue-specific Smarca5 deletions, e.g., in the hematopoietic system. Here we show that, in a gene dosage-dependent manner, the hypomorphic allele of SMARCA5 (S5tg) can rescue not only the developmental arrest in hematopoiesis in the hCD2iCre model but also the lethal phenotypes associated with constitutive Smarca5 deletion or Vav1iCre-driven conditional knockout in hematopoietic progenitor cells. Interestingly, the latter model also provided evidence for the role of SMARCA5 expression level in hematopoietic stem cells, as the Vav1iCre S5tg animals accumulate stem and progenitor cells. Furthermore, their hematopoietic stem cells exhibited impaired lymphoid lineage entry and differentiation. This observation contrasts with the myeloid lineage which is developing without significant disturbances. Our findings indicate that animals with low expression of SMARCA5 exhibit normal embryonic development with altered lymphoid entry within the hematopoietic stem cell compartment.

2025-10-27 | PXD048865 | panorama

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Project description:Fosl1 transdifferentiate embryonic stem cells to differentiated trophoblast stem cells. (ChIP-seq)

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Mus musculus

Project description:Fosl1 transdifferentiate embryonic stem cells to differentiated trophoblast stem cells (RNA-seq)

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Project description:ChIP-seq anlysis of Sox2, Tfap2c, and Cdx2 in trophoblast stem cells.

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Project description:Trophoblast stem cells