Homo sapiens
Ontology highlight
ABSTRACT:
OTHER RELATED OMICS DATASETS IN: E-GEOD-35006
PROVIDER: PRJNA151175 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Profiling of p53-responsive genes in human breast cancer cells harboring endogenous ts-p53 E285K
2025-01-24 | E-GEOD-35006 | ExpressionAtlas
Project description:The ts-p53 E285K protein is a rare p53 mutant with temperature-sensitive (ts) loss of function characteristics. In cancer cells, which express ts-p53 E285K intrinsically, endogenous wild type p53 activity is reconstituted by appropriate cultivation temperature (permissive condition). At non-appropriate cultivation temperature (restrictive condition) this p53 mutant is inactive. The present study took advantage of this mechanism and employed IPH-926 lobular breast cancer cells and BT-474 ductal breast cancer cells, which both harbor endogenous ts-p53 E285K, for the transcriptional profiling of p53-responsive genes. This new approach eliminated the need for genetic modification or cytotoxic stimulation to achive a p53 response in the cells being investigated . Three subseqent passages of IPH-926 lobular breast cancer cells (harboring ts-p53 E285K) were seeded into two parallel culture dishes each and were allowed to adopt to restrictive and permissive condition for 24 h before analysis on Affymetrix U133 Plus 2.0 microarrays. Subsequently, this experiment was repeated with BT-474 ductal breast cancer cells (also harboring ts-p53 E285K). To gate out non-specific temperature effects, the same experiment was also performed with MCF-7 breast cancer cells (harboring wt p53). Probe sets differentially expressed at restrictive versus permissive condition in MCF-7 were considered as non-specifically regulated. These probe sets were excluded from the final statistical analysis of IPH-926 and BT-474 expression data. response to restored p53 activity
2012-12-31 | E-GEOD-35006 | biostudies-arrayexpress
Project description:The ts-p53 E285K protein is a rare p53 mutant with temperature-sensitive (ts) loss of function characteristics. In cancer cells, which express ts-p53 E285K intriniscally, endogenous wild type p53 activity is reconstituted by appropriate cultivation temperature (permissive condition). At non-appropriate cultivation temperature (restrictive condition) this p53 mutant is inactive. The present study took advantage of this mechanism and employed IPH-926 lobular breast cancer cells and BT-474 ductal breast cancer cells, which both harbor endogenous ts-p53 E285K, for the transcriptional profiling of p53-responsive genes. This new approach eliminated the need for genetic modification or cytotoxic stimulation to achive a p53 response in the cells being investigated .
2012-12-31 | GSE35006 | GEO
Project description:Transcription profiling by array of human breast cancer mammospheres and breast tumors
2025-01-24 | E-GEOD-7515 | ExpressionAtlas
Project description:Transcription profiling of human pre-malignant breast cancer samples
2025-01-24 | E-GEOD-7377 | ExpressionAtlas
Project description:Transcription profiling by array of human hormone-responsive MCF-7 cells versus estrogen-deprived breast cancer cells
2025-01-16 | E-GEOD-10879 | ExpressionAtlas
Project description:Transcription profiling by array of human MCF-7 breast cancer cells
2025-01-16 | E-GEOD-11791 | ExpressionAtlas
Project description:Resveratrol induces downregulation of DNA repair genes in MCF7 human breast cancer cells.
2025-01-24 | E-GEOD-25412 | ExpressionAtlas
Project description:Transcription profiling by array of human breast cancer cell lines treated with fulvestrant
2025-01-24 | E-GEOD-22533 | ExpressionAtlas
Project description:Networking of differentially expressed genes in human MCF7 breast cancer cells resistant to methotrexate
2025-01-24 | E-GEOD-16070 | ExpressionAtlas