Project description:We used microarrays to compare interferon-alpha (IFNa)- and interferon-gamma (IFNg)-stimulated genes under an equivalent biological input. The goal was to compare IFNa- and IFNg-stimulated genes, as well as to identify common and distinct sets of type I and II ISGs. Bone marrow macrophages derived from mouse bone marrow in M-CSF for 7 days. The cells were stimulated with 62U/mL IFNa and 1U/mL of IFNg for 2.5 hrs in culture. These concentrations induced equivalent STAT1 phosphorylation in BMMs.

2012-02-15 | E-GEOD-35825 | biostudies-arrayexpress

E-GEOD-42257

Project description:Murine bone marrow gene expression profiling

2015-08-06 | E-GEOD-42257 | ExpressionAtlas

E-GEOD-63653

Project description:Expression data from bone marrow-derived macrophages

2016-05-03 | E-GEOD-63653 | ExpressionAtlas

Histoplasma capsulatum induces a Type I interferon response that contributes to pathogenesis in mice

Project description:To identify signaling pathways that are induced by macrophages infected with Histoplasma capsulatum, we examined the whole genome expression profile of murine bone marrow derived macrophages infected with conidia, the natural infectious particle of Histoplasma. Conidia induced the expression of signature Type I interferon response genes. The induction of a Type I interferon response was specific to conidia, yeast cells did not trigger the response. Macrophages that lack the Type I interferon receptor, IFNAR1, were infected and compared to wild-type macrophages. The expression of some Type I IFN response genes are dependent upon Keywords: Murine bone marrow derived macrophage transcriptional response to infection with Histoplasma capsulatum conidia We analyzed a series of 24 MEEBO arrays on which were hybed RNA amplified from bone marrow derived macrophages from C57BL/6 (WT) or ifnar1-/- mice either mock infected or infected with H. capsulatum conidia or yeast cells.

2010-05-06 | E-GEOD-20022 | biostudies-arrayexpress

Identifying interactors of IRF1 and STAT1 during type I and type II interferon treatment using proximity-dependent labeling

Project description:Identifying interactors of IRF1 and STAT1 in bone marrow-derived macrophages during type I and type II interferon treatment using the proximity-dependent labeling approach TurboID followed by Mass Spectrometry

2024-03-12 | PXD040337 | Pride

Conidia but not yeast cells of the fungal pathogen Histoplasma capsulatum trigger a type I interferon innate immune response in murine macrophages

2010-03-01 | GSE20022 | GEO

E-GEOD-22434

Project description:Expression data from Evi1-transduced primary bone marrow cells

2015-09-15 | E-GEOD-22434 | ExpressionAtlas