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Arabidopsis thaliana

ABSTRACT: Nucleotidyltransferase gene HESO1

PROVIDER: PRJNA152365 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR403903.fastq.gz	Fastqsanger.gz
	SRR403904.fastq.gz	Fastqsanger.gz
	SRR403905.fastq.gz	Fastqsanger.gz
	SRR403906.fastq.gz	Fastqsanger.gz
	SRR403907.fastq.gz	Fastqsanger.gz

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Arabidopsis thaliana

Project description:Distinct and cooperative activities of HESO1 and URT1 nucleotidyl transferases in microRNA turnover in Arabidopsis

| PRJNA260855 | ENA

Distinct and cooperative activities of HESO1 and URT1 nucleotidyl transferases in microRNA turnover in Arabidopsis

Project description:3’ uridylation is increasingly recognized as a conserved RNA modification process associated with RNA turnover in eukaryotes. 2’-O-methylation on the 3’ terminal ribose protects micro(mi)RNAs from 3’ truncation and 3’ uridylation in Arabidopsis. Previously, we identified HESO1 as the nucleotidyl transferase that uridylates most unmethylated miRNAs in vivo, but substantial 3’ tailing of miRNAs still remains in heso1 loss-of-function mutants. In this study, we found that among nine HESO1 paralogs, UTP:RNA URIDYLYLTRANSFERASE 1 (URT1) is the single most predominant nucleotidyl transferase that tails miRNAs. URT1 and HESO1 prefer substrates with different 3’ end nucleotides in vitro and act cooperatively to tail different forms of the same miRNAs in vivo. Moreover, both HESO1 and URT1 exhibit nucleotidyl transferase activity on AGO1-bound miRNAs. Although these enzymes are able to add long tails to AGO1-bound miRNAs, the tailed miRNAs remain associated with AGO1. In fact, we show that a tailed miRNA acquires the ability to trigger the production of secondary siRNAs. Therefore, 3’ uridylation could endow new properties to miRNAs in addition to its known effects in miRNA degradation.

2015-08-19 | GSE61362 | GEO

Prevalent cytidylation and uridylation of precursor miRNAs in Arabidopsis

Project description:A key step in microRNA (miRNA) biogenesis is the processing of a primary precursor RNA by the microprocessor into a precursor miRNA (pre-miRNA) intermediate. In plants, little is known about the processes that act on pre-miRNAs to influence miRNA biogenesis. Here, we performed 3’ RACE-seq to profile pre-miRNA 3’ ends in Arabidopsis. 3’ end heterogeneity was prevalent and the three microprocessor components promoted 3’ end precision. Extensive cytidylation and uridylation of precise and imprecise pre-miRNA 3’ ends were uncovered. The nucleotidyl transferase HESO1 uridylated pre-miRNAs in vitro and was responsible for most pre-miRNA uridylation in vivo. HESO1, NTP6 and NTP7 contribute to pre-miRNA cytidylation. Tailing of pre-miRNAs tended to restore trimmed pre-miRNAs to intact lengths to promote further processing. In addition, HESO1-mediated uridylation led to the degradation of some imprecisely processed pre-miRNAs. Thus, we uncovered widespread cytidylation and uridylation of pre-miRNAs and demonstrated diverse functions of pre-miRNA tailing in plants.

2019-10-22 | GSE134124 | GEO

Synergistic and independent actions of multiple terminal nucleotidyl transferases in 3’ tailing of small RNAs in Arabidopsis

Project description:Small silencing RNAs are key regulators of gene expression in both plants and animals. HEN1-mediated 3’ terminal 2’-O-methylation plays a crucial role in small RNA stability control. In the absence of HEN1, small RNAs are frequently uridylated (untemplated uridine addition) and trimmed, a phenomenon that is conserved across species. However, the underlying molecular mechanism is largely unknown. In this study, we have discovered UTP: RNA uridylyltransferase (URT1) acts redundantly with HESO1 in the uridylation of miRNAs, in addition to its role in oligo-adenylated mRNA uridylation. We show both common and distinctive features of URT1 and HESO1 in catalyzing miRNA uridylation.

2015-07-01 | GSE60826 | GEO

Nucleotidyltransferase gene HESO1

Project description:Small RNAs in plants are protected by 2'-O-methylation at 3' terminal nucleotide by HEN1. In hen1 mutant, small RNAs lacking 3' methylation are truncated and tailed at 3' end. We identified a nucleotidyltransferase gene HESO1 in Arabidopsis that tails small RNAs in hen1. In order to study the small RNA tailing and truncation in detail in wild type Col, heso1-1, hen1-8 and hen1-8 heso1-1 double mutant, we constructed small RNA libraries for these four genotypes and we found heso1-1 mutation reduces small RNA tailing in hen1-8 background, however, the 3' truncation is not significantly affected. 8 samples examined: wild-type Col (replicate 1), heso1-1 (replicate 1), hen1-8(replicate 1), hen1-8 heso1-1 (replicate 1), wild-type Col (replicate 2), heso1-1 (replicate 2), hen1-8(replicate 2) and hen1-8 heso1-1 (replicate 2).

2012-06-30 | E-GEOD-35479 | biostudies-arrayexpress

Nucleotidyltransferase gene HESO1

2012-07-01 | GSE35479 | GEO

Involvement of Arabidopsis TUTases in viral RNA uridylation

Project description:Using 3' RACE-seq method, we analyzed the impact of URT1 and/or HESO1 inactivation on the uridylation of GFLV, TuMV and TCV RNAs in Arabidopsis.

2023-04-27 | GSE212293 | GEO

Synergistic and independent actions of multiple terminal nucleotidyl transferases in 3’ tailing of small RNAs in Arabidopsis

2015-07-01 | E-GEOD-60826 | biostudies-arrayexpress

Impact of URT1 and HESO1 TUTases on the steady state level of mRNAs in Arabidopsis seeds and flowers

Project description:To evaluate the contribution of both TUTases on the mRNA steady state level, we produced total RNA-seq libraries from flowers and dry seeds of WT plants and of mutant for URT1 and HESO1. We performed a differential gene expression analysis to identify mRNAs that are differentially accumulated in mutant seeds and flowers when compared to WT

2025-01-13 | GSE284704 | GEO