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Homo sapiens

ABSTRACT: Genome-wide maps of CtBP binding profile in breast cancer cell line MCF-7

PROVIDER: PRJNA155587 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR445955.fastq.gz	Fastqsanger.gz
	SRR445956.fastq.gz	Fastqsanger.gz
	SRR445957.fastq.gz	Fastqsanger.gz

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Genome-wide maps of CtBP binding profile in breast cancer cell line MCF-7

Project description:CtBP is a global co-repressor by serving as transcriptional factor in multiple pathways. CtBP functioned as transcriptional factor by recruiting other cofactors such as G9a, HDAC1 and PcG proteins. CtBP is found to be over enriched in several type of tumor samples. To dipict the role of CtBP in globally regulating gene expression, we applied ChIP-seq technology to find out the binding profile of CtBP in breast cancer cell line MCF-7. Our data suggest CtBP plays a global regulatory role in DNA damage repair, tumor initiation, and EMT process. Examine CtBP binding in MCF-7 cells

2013-03-20 | E-GEOD-36546 | biostudies-arrayexpress

Genome-wide maps of CtBP binding profile in breast cancer cell line MCF-7

2013-03-20 | GSE36546 | GEO

Effect of kncoking down CtBP and TRIM28 on gene expreession in MCF-7 cells

Project description:Both TRIM28 and CtBP are important transcriptional repressors and always shown high expression in many types of cancers, in our current study, we found TRIM28 interacts with CtBP in breast cancer and normal cells. To elucidate the functions of interaction between TRIM28 and CtBP in breast cancer progression, we performed the RNAseq of knocking down TRIM28, CtBP and Ctrl(NC) genes in MCF-7 cell line.

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Project description:Previously, we have shown that HIST1H2ac is overexpressed in MCF-7 breast cancer cell line. It acts as a master regulator of estrogen receptor alpha-dependent gene expression in ER+ breast cancer cells. In the present study, we investigate the genome-wide protein DNA-binding events of HIST1H2ac protein in MCF-7 breast cancer cell line by over-expressing hemagglutinin (HA)-tagged HIST1H2ac and compared with MCF-7 cells over-expressing HA. The protein-bound DNA was recovered by immunoprecipitation using anti-HA antibody. The ChIP DNA and input DNA were sequenced with an Illumina HiSeq 2000 sequencer.

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Project description:INPP4B over-expressed INPP4B MCF-7 luminal breast cancer cell line

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Project description:Genome-wide maps of TRIM25 binding sites in triple negative breast cancer cell

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Project description:Genome sequencing from MCF-7 breast cancer cell line

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Project description:We studied genes, that are differentially expressed between malignant and normal breast tissue, to find weak spots for anti-cancer therapy development. RNA sequencing of three cell lines was performed: MCF-7 (epithelial breast cancer cell line), BCC (primary breast tumour cell line) and MCF-10A (epithelial breast cell line).

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