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Arabidopsis thaliana

ABSTRACT: An IDN2-containing complex involved in RNA-directed DNA methylation in Arabidopsis [leaves RNA-seq]

PROVIDER: PRJNA155791 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR419182.fastq.gz	Fastqsanger.gz
	SRR419183.fastq.gz	Fastqsanger.gz
	SRR419184.fastq.gz	Fastqsanger.gz
	SRR419185.fastq.gz	Fastqsanger.gz
	SRR419186.fastq.gz	Fastqsanger.gz

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An IDN2-containing complex involved in RNA-directed DNA methylation in Arabidopsis

Project description:This SuperSeries is composed of the following subset Series: GSE36129: An IDN2-containing complex involved in RNA-directed DNA methylation in Arabidopsis [leaves RNA-seq] GSE36143: An IDN2-containing complex involved in RNA-directed DNA methylation in Arabidopsis [BS-seq] GSE37206: An IDN2-containing complex involved in RNA-directed DNA methylation in Arabidopsis [flowers RNA-seq] Refer to individual Series

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Project description:We explored the mechanism by which RdDM affects nucleosome positioning in Arabidopsis thaliana. We showed that POLV has a direct effect on nucleosomes through the SWI/SNF complex. We found that the AGO4-siRNA complex is involved in nucleosome positioning via IDN2. Moreover, the SWI/SNF complex is not required for DNA methylation in positioned nucleosomes. Instead, we found that DNA methylation is needed for nucleosome positioning in differentially methylated regions. Taken together, we propose a model where the RdDM pathway directs nucleosome positioning through DNA methylation to establish transcriptional gene silencing.

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