Bos indicus
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ABSTRACT:
PROVIDER: PRJNA164685 | ENA |
REPOSITORIES: ENA
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Project description:Equine chorionic gonadotropin (eCG) is a widely used hormone to synchronize the female cycle and induce estrusestruses in livestock breeding. eCG is a heterodimeric glycoprotein composed of non-covalently linked α- and β-chains whose glycosylation profiles determine the in vivo activity of the hormone. The commercially available eCG products are crudely purified from the serum of pregnant mares, hence called pregnant mare serum gonadotropin (PMSG). A correct glycosylation of the protein is crucial for the correct binding of the receptor, the half-life of the hormone and the intended function as such. The exact protein composition of the various commercial PMSG products and their specific glycosylation pattern have not been characterized so far. Therefore, in this study, we used proteomic approaches to analyze and compare four PMSG products. Here we show that the examined PMSGs share a surprisingly low level of commonalities (5.5%) in protein composition among the four tested products, with serum proteins as the main variable. Analysing the site-specific N-glycosylation, we identified for the first time the presence of O-acetylation of sialic acid at the structure of the glycan of eCG, which we could not find in significant amounts on human CG suggesting that this modification is species-specific.
2025-05-06 | PXD042535 | Pride
Project description:Small RNA analysis of bovine corpus luteum
| PRJNA263410 | ENA
Project description:In order to indentify genes regulated by eCG, and involved in CL development and progesterone increases, the transcriptome was evaluated using the microarray technology Cows (Bos indicus) were divided into control, stimulated and superovulated groups. The stimulated group received 400 IU of eCG on day 8 and the superovulated group received 2000 IU of eCG on day 4 after the beginning of synchronization. Corpus luteum were collected at day 6 after ovulation e the trasncripitome was available by microarray.
2012-05-08 | E-GEOD-37844 | biostudies-arrayexpress
Project description:Follicle stimulating hormone (FSH) and epidermal growth factor (EGF) are currently used on cumulus-oocyte complexes to mimic the luteinizing hormone surge in vitro and induce oocyte maturation and cumulus expansion. We have previously shown that addition of exogenous recombinant growth differentiation factor 9 (GDF9) during oocyte in vitro maturation led to an improvement of oocyte quality, as shown by an increased blastocyst percentage and fetal survival. Our objective was to characterize the effect of FSH/EGF and GDF9 treatments on mouse cumulus cells expression profile by microarray analysis. Cumulus-oocyte complexes (COCs) were recovered from 21 to 26 day old female 129/SV mice, 44 hours post equine chorionic gonadotropin treatment (eCG (5 IU)). For the microarray experiment whole COCs were treated with 293H control medium (0.125% v/v), with 20 ng/ml GDF9 or with a combination of 50 mIU/ml FSH and 10 ng/ml EGF. After 8 hours of in vitro maturation, COCs were denuded by gentle pipetting, the oocytes were removed and the cumulus cells centrifuged and extracted RNA analysed by microarray.
2011-12-10 | E-GEOD-19327 | biostudies-arrayexpress
Project description:Microarray analysis of the transcriptome in the primate corpus luteum during chorionic gonadotropin administration simulating early pregnancy.
| PRJNA134863 | ENA
Project description:Prostaglandin F2α -induced changes in transcriptome of bovine mid cycle versus early corpus luteum
| PRJNA131457 | ENA
Project description:<p>Introduction: Gonadotropin-releasing hormone (GnRH) is widely used in the timed artificial insemination program of sheep industry, but there is still controversy about the effect of GnRH on pregnancy rate in the process of artificial insemination. Our research shows that the use of GnRH reduces the pregnancy rate. This study aims to analyze the metabolite changes caused by GnRH in Huyang ewes before the implantation period through metabolomics technology, and reveal the mechanism behind the decreased pregnancy rate. </p><p>Methods: All ewes had a polyurethane sponge containing 45 mg of flurogestone acetate placed in their vagina for 12 days, and were intramuscularly treated with 330 units of equine chorionic gonadotropin (eCG) immediately after sponge removal. The experimental group (n = 69) received an intramuscular treatment of 17 μg GnRH agonist triptorelin (Day 0) 48 h after sponge removal, while the control group (n = 41) was treated with 1 mL of sterile saline. All ewes underwent a single vaginal insemination 58 h after the withdrawal of progesterone sponges. The difference in pregnancy rates between the two groups was then measured. Metabolomic analysis was performed on plasma samples collected on Day 7. </p><p>Results: Compared with the control group, GnRH treatment significantly reduced the pregnancy rate in the experimental group (72.2% vs 82.9%, P < 0.05). Metabolomic analysis revealed that GnRH treatment influenced metabolites involved in collagen synthesis and metabolites linked to prostaglandin synthesis in the endometrial tissue, which includes a marked decrease in hydroxyproline metabolite content and a substantial increase in corticosterone and prostaglandin D2 metabolite levels. </p><p>Conclusion: In summary, GnRH agonist Triptorelin injected 48 h after the withdrawal of progesterone sponges can reduce the pregnancy rate of Huyang ewe after artificial insemination and affect the metabolite levels related to endometrial collagen synthesis and prostaglandin synthesis, which are not conducive to embryo implantation.</p>
2024-08-12 | MTBLS10318 | MetaboLights
Project description:A surge of luteinizing hormone (LH) from the pituitary gland triggers ovulation, oocyte maturation, and luteinization for successful reproduction in mammals. Since the signaling molecules RAS and ERK1/2 are activated by a LH surge in granulosa cells of preovulatory follicles, we disrupted Erk1/2 in mouse granulosa cells and provide in vivo evidence that these kinases are necessary for LH-induced oocyte resumption of meiosis, ovulation, and luteinization. In addition, biochemical analyses and selected disruption of the Cebpb gene in granulosa cells demonstrate that C/EBP is a critical downstream mediator of ERK1/2 activation. These mouse models provide in vivo systems in which to define the context specific and molecular mechanisms by which granulosa cells respond to LH and these mechanisms are relevant to the regulation of human fertility and infertility. Immature wild type or ERK1/2 conditonal knock-out mice were injected with 5IU equine chorionic gonadotropin (eCG)-48h followed by 5 IU hCG injection. The ovarian granulosa cells were collected at hCG 0h, 2.5h, or 4h and the gene expression pforiles were compared by microarray method.
2009-03-20 | E-GEOD-15315 | biostudies-arrayexpress
Project description:Follicle stimulating hormone (FSH) regulates ovarian follicular development through a specific gene expression program. We analyzed FSH-regulated transcriptome and histone modification in granulosa cells during follicular development. We used super-stimulated immature mice and collected granulosa cells prior to and 48h after stimulation with equine chorionic gonadotropin (eCG). We profiled the transcriptome using RNA-sequencing (N=3/time-point) and genome wide trimethylation of lysine 4 of histone H3 (H3K4me3; an active transcription marker) using chromatin immunoprecipitation and sequencing (ChIP-Seq; N=2/time-point). Across the mouse genome 14,583 genes had an associated H3K4me3 peak and 63-66% of these peaks were observed within < 1kb promoter region. There were 72 genes with differential H3K4me3 modification at 48h-eCG (absolute log fold change >1; FDR<0.05) relative to 0h-eCG. Transcriptome data analysis showed 1463 differentially expressed genes at 48h-eCG (absolute log fold change > 1; FDR<0.05). Among the 20 genes with differential expression and altered H3K4me3 modification, Lhcgr had higher H3K4me3 abundance and expression, while Nrip2 had lower H3K4me3 abundance and expression. Using ChIP-qPCR, we showed that FSH regulated expression of Lhcgr, Cyp19a1, Nppc and Nrip2 through regulation of H3K4me3 at their respective promoters. Transcript isoform analysis using Kallisto-Sleuth tool revealed 875 differentially expressed transcripts at 48h-eCG (b>1; FDR<0.05). Pathway analysis of RNA-seq data demonstrated that TGFB signaling and steroidogenic pathways were regulated at 48h-eCG. Thus, FSH regulates gene expression in granulosa cells through multiple mechanisms namely altered H3K4me3 modification and inducing specific transcripts. These data form the basis for further studies investigating how these specific mechanisms regulate granulosa cell functions.
2020-09-07 | GSE140371 | GEO
Project description:Obesity leads to ovarian dysfunction and the establishment of local leptin resistance. The aim of our study was to characterise levels of Nod-Like Receptor Protein 3 (NLRP3) inflammasome activation during obesity progression in the mouse ovaries and liver and test the putative role of leptin on its regulation. C57BL/6J mice were treated with equine chorionic gonadotropin (eCG) or human chorionic gonadotropin (hCG) for oestrous cycle synchronisation and ovaries collection. In diet-induced obesity (DIO) model, mice were fed chow diet (CD) or high fat diet (HFD) for 4 or 16 weeks (wk), whereas in hyperleptinemic model (LEPT), mice were injected with leptin for 16 days (16L) or saline (16C) and in the genetic obese leptin-deficient ob/ob (+/? and -/-) animals were fed CD for 4wk. Either ovaries and liver were collected, as well as cumulus cells (CCs) after superovulation from DIO and LEPT. In DIO protocol, protein expression of NLRP3 inflammasome components was increased in 4wk HFD, but decreased in 16wk HFD. Moreover LEPT and ob/ob models revealed NLRP3 and IL-1 upregulation in 16L and downregulation in ob/ob. Transcriptome analysis of CC showed common genes between LEPT and 4wk HFD modulating NLRP3 inflammasome. Moreover analysis in the liver showed upregulation of NLRP3 protein only after 16wk HFD, but also the downregulation of NLRP3 protein in ob/ob-/-. We showed the link between leptin signalling and NLRP3 inflammasome activation in the ovary throughout obesity progression in mice, elucidating the molecular mechanisms underpinning ovarian failure in maternal obesity.
2021-11-01 | GSE180300 | GEO