Project description:We experimentally manipulate CO2 levels and investigate the effects of high-CO2/low pH on seagrass metabolism and underlying molecular mechanisms. Cymodocea nodosa plants were collected in Cadiz Bay at the end of January 2014 and transported to the mesocosm facility. After a one week acclimation period, C. nodosa were either kept under normal (400 ppm) or elevated (1200 ppm) CO2 concentration for 12 days. RNA extraction and sequencing was performed in all phases to test differential expression of genes in different conditions.
Project description:This study investigated the transcriptomic response of Paraburkholderia busanensis P39 during exposure to fungal biomass of Colletotrichum scovillei KC05. RNA-seq was used to compare P39 grown alone with P39 exposed to fungal biomass, with three biological replicates per condition. The study aimed to identify transcriptional changes associated with fungal-biomass adaptation, nutrient scavenging, cell-envelope remodeling, regulatory responses, and interface-associated functions. Reads were aligned to the Paraburkholderia busanensis P39 genome represented by GenBank accessions CP058248 and CP058249.
Project description:Investigation of whole genome gene expression level changes in Lactococcus lactis KCTC 3769T,L. raffinolactis DSM 20443T, L. plantarum DSM 20686T, L. fujiensis JSM 16395T, L. garvieae KCTC 3772T, L. piscium DSM 6634T and L. chungangensis CAU 28T . This proves that transcriptional profiling can facilitate in elucidating the genetic distance between closely related strains.