Project description:The mucosal surfaces of fish serve as the first-line of defense against the myriad of aquatic pathogens present in the aquatic environment. The immune repertoire functioning at these interfaces is still poorly understood. The skin, in particular, must process signals from several fronts, sensing and integrating environmental, nutritional, social, and health cues. Pathogen invasion can disrupt this delicate homeostasis with profound impacts on signaling throughout the organism. Here, we investigated the transcriptional effects of virulent A. hydrophila infection in channel catfish skin, Ictalurus punctatus. We utilized an 8X60K Agilent microarray to examine gene expression profiles at critical early timepoints following challenge—2 h, 8 h, and 12 h. Expression of a total of 2,168 unique genes was significantly perturbed during at least one timepoint. We observed dysregulation of a number of genes involved in antioxidant, cytoskeletal, immune, junctional, and nervous system pathways. In particular, A. hydrophila infection rapidly altered a number potentially critical lectins, chemokines, interleukins, and other mucosal factors in a manner predicted to enhance its ability to adhere and invade the catfish host.
Project description:The succinylated modification on bacterial protein lysine residues is currently reported to be crucial for regulating cellular physiology and pathology. In this study, to investigate the effect of the lysine succinylation modification on the biological regulation in a well-known fish pathogen, Aeromonas hydrophila, a highly affinity purification was performed to enrich lysine succinylation peptides in A. hydrophila and then identified a total 2174 lysine succinylation sites on 666 proteins with LC-MS/MS. The further motif analysis showed eight motifs surrounding the central lysine succinylated residue were conservative which share some common preferences with other bacterial species. Gene ontology analysis showed that these succinylated proteins involved in diverse metabolic pathways and biological processes, such as translation, protein export and central metabolic pathways. In general, our study provides a significant insight into the functions of lysine succinylation in the cellular physiology and pathology in A.hydrophila.