Project description:TIE1 regulates leaf development by repressing leaf differentiation because the semi-dominant mutant tie1-D by activation tagging displays small and hyponastic leaves and the differentiation of leaf epidermal cells is delayed in the tie1-D mutant, whereas disruption of TIE1 causes epinastic leaves with early differentiated epidermal cells. We used microarrays to investigate the molecular base underpinning the phenotypes of TIE1-overexpressing plants. Aerial parts of 14-day-old seedlings from wild-type and GFP-TIE1-15 were collected for RNA extraction and hybridization on Affymetrix microarrays.
Project description:Transcription profiling of aerial parts of Arabidopsis wild type and arr10 arr12 double mutant seedlings treated with the cytokinin trans-zeatin
Project description:(1) Phosphopeptide abundances of group B-Rafs, SnRK2s or SnRK2 substrates in the aerial parts of soil-grown Arabidopsis wild-type (Col-0) seedlings with/without water supply for indicated periods. Data were obtained from three independent biological replicates. (2)Phosphopeptide abundances in 2-week-old Arabidopsis wild-type (Col-0) seedlings treated with/without dehydration on the filter paper for 30 min. Data were obtained from three independent biological replicates. (3) Phosphopeptide abundances in 2-week-old Arabidopsis wild-type (Col-0), raf13-1raf15-1 or ireh1-2 seedlings under optimal growth conditions. Data were obtained from three independent biological replicates.
Project description:Gene expression profiles in 15 day old low oil 1 (loo1) mutant and the wild type A15 seedlings were compared. Keywords: Comparison and loo1 and wild type seedling transcriptomes
Project description:RNA-seq of Arabidopsis 14 day-old seedlings expressing either mCherry-RAX1-GR or mCherry-GR with 4h mock vs. dexamethasone treatment
Project description:Receptor-like kinases (RLKs) play important roles in plant development and defense responses; however, their functions in other processes remain unclear. Here, we report that OsSIK2, an S-domain RLK from rice, is involved in abiotic stress and senescence process, integrating stress signals into developmental program for adaptive growth. We used microarrays to detail the gene expression in OsSIK2-overexpression and mutant lines from 14-day-old rice seedlings and identified distinct regulated genes. We sought to obtain distinct regulated genes by OsSIK2. To that end,the aerial parts of different rice seedlings,including OsSIK2-overexpression lines, mutant and respective controls, were harveted for total RNA extraction and hybridization on Affymetrix microarrays.